González-Castillo Adrián, Balboa Sabela, Romalde Jesús L, Gomez-Gil Bruno
CIAD, A.C. Mazatlán Unit for Aquaculture, AP. 711, 82000, Mazatlán, Sinaloa, Mexico.
Antonie Van Leeuwenhoek. 2014 Sep;106(3):457-63. doi: 10.1007/s10482-014-0214-4. Epub 2014 Jun 28.
A motile, facultative anaerobic, marine bacterial isolate (CAIM 1437(T)) was obtained from a cultured oyster (Crassostrea gigas) in Sonora, México. The strain was studied by a phylogenetic analysis based on sequences of the 16S rRNA and five housekeeping genes, i.e. ftsZ, gapA, pyrH, recA, and topA. Comparison of the almost-complete 16S rRNA gene sequence with those of other type strains of the genus Vibrio showed a close relationship with the type strains of Vibrio orientalis and Vibrio rotiferianus, with similarity values ranging from 98.4 to 98.3 %, respectively. MLSA placed this strain within the Orientalis clade. The DNA-DNA hybridization value of strain CAIM 1437(T) with V. orientalis was 59 % and with V. rotiferianus 55 %. The DNA G+C content was determined to be 45.6 mol %. Phenotypic characteristics also showed differences with the species analysed. The results presented here support the description of a novel species, for which the name Vibrio crosai sp. nov. is proposed, with CAIM 1437(T) (= DSM 27145(T)) as the type strain.
从墨西哥索诺拉州养殖的牡蛎（太平洋牡蛎）中获得了一株运动性、兼性厌氧的海洋细菌分离株（CAIM 1437(T)）。基于16S rRNA和五个管家基因（即ftsZ、gapA、pyrH、recA和topA）的序列，通过系统发育分析对该菌株进行了研究。将几乎完整的16S rRNA基因序列与弧菌属其他模式菌株的序列进行比较，结果表明它与东方弧菌和轮虫弧菌的模式菌株关系密切，相似性值分别为98.4%至98.3%。多位点序列分析将该菌株置于东方弧菌进化枝内。菌株CAIM 1437(T)与东方弧菌的DNA-DNA杂交值为59%，与轮虫弧菌为55%。测定DNA G+C含量为45.6 mol%。表型特征也显示出与所分析物种的差异。此处给出的结果支持描述一个新物种，为此提出名为克氏弧菌（Vibrio crosai sp. nov.），以CAIM 1437(T)（= DSM 27145(T)）作为模式菌株。
