Department of Forensic Medicine and Pathology, University Hospital R. Poincaré (AP-HP, UVSQ), 104 R. Poincaré boulevard, 92380 Garches, France.
Forensic Sci Int. 2013 Mar 10;226(1-3):38-40. doi: 10.1016/j.forsciint.2012.11.018. Epub 2012 Dec 30.
A mummified head was identified in 2010 as belonging to Henri IV, King of France. A putative blood sample from the King Louis XVI preserved into a pyrographically decorated gourd was analyzed in 2011. Both kings are in a direct male-line descent, separated by seven generations. We have retrieved the hypervariable region 1 of the mitochondrial DNA as well as a partial Y-chromosome profile from Henri IV. Five STR loci match the alleles found in Louis XVI, while another locus shows an allele that is just one mutation step apart. Taking into consideration that the partial Y-chromosome profile is extremely rare in modern human databases, we concluded that both males could be paternally related. The likelihood ratio of the two samples belonging to males separated by seven generations (as opposed to unrelated males) was estimated as 246.3, with a 95% confidence interval between 44.2 and 9729. Historically speaking, this forensic DNA data would confirm the identity of the previous Louis XVI sample, and give another positive argument for the authenticity of the head of Henri IV.
2010 年，一个木乃伊化的头颅被鉴定为法国国王亨利四世所有。2011 年，对保存在一个火绘装饰葫芦里的疑似路易十六国王的血液样本进行了分析。这两位国王是直系男性后裔，相隔七代。我们从亨利四世那里提取了线粒体 DNA 的高变区 1 以及部分 Y 染色体图谱。有五个 STR 基因座与路易十六的等位基因匹配，而另一个基因座的等位基因只有一个突变步骤的差异。考虑到部分 Y 染色体图谱在现代人类数据库中极为罕见，我们得出结论，这两个男性可能存在父子关系。两个样本属于相隔七代的男性（而不是无关男性）的似然比估计为 246.3，95%置信区间在 44.2 到 9729 之间。从历史上看，这些法医 DNA 数据将证实之前路易十六样本的身份，并为亨利四世头颅的真实性提供另一个有力证据。
