Servais Anne-Catherine, Fillet Marianne
Department of Pharmaceutical Sciences, University of Liège, Liège, Belgium.
Methods Mol Biol. 2013;970:297-305. doi: 10.1007/978-1-62703-263-6_19.
The enantioseparation of acidic and basic compounds can be successfully achieved in nonaqueous capillary electrophoresis using single-isomer charged β-cyclodextrin (β-CD) derivatives of opposite charge to that of the analytes. This chapter describes how to separate the enantiomers of three basic substances selected as model compounds, i.e., alprenolol, bupranolol, and terbutaline, using the negatively charged heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-CD. The enantiomers of three acidic drugs (tiaprofenic acid, suprofen, and flurbiprofen) are resolved using a monosubstituted amino β-CD derivative, namely, 6-monodeoxy-6-mono(3-hydroxy)propylamino-β-CD.
在非水毛细管电泳中，使用与分析物电荷相反的单异构体带电β-环糊精（β-CD）衍生物，可以成功实现酸性和碱性化合物的对映体分离。本章描述了如何使用带负电荷的七（2,3-二-O-乙酰基-6-O-磺基）-β-CD分离三种被选作模型化合物的碱性物质（即阿普洛尔、布普洛尔和特布他林）的对映体。三种酸性药物（噻洛芬酸、舒洛芬和氟比洛芬）的对映体则使用单取代氨基β-CD衍生物，即6-单脱氧-6-单（3-羟基）丙基氨基-β-CD进行拆分。
