Servais Anne-Catherine, Fillet Marianne
Department of Pharmaceutical Sciences, University of Liège, Liège, Belgium.
Methods Mol Biol. 2013;970:289-95. doi: 10.1007/978-1-62703-263-6_18.
The enantioseparation of acidic and neutral compounds can be successfully achieved in capillary electrophoresis (CE) using dual cyclodextrin (CD) systems. This chapter describes how to separate the enantiomers of acidic or neutral substances using dual CD systems made up of a negatively charged CD derivative, i.e., sulfobutyl-β-CD (SB-β-CD) or carboxymethyl-β-CD (CM-β-CD), in combination with a neutral one, namely, heptakis(2,3,6-tri-O-methyl)-β-CD (TM-β-CD). An acidic compound (carprofen) and a weakly acidic drug (pentobarbital) were selected as model compounds.
使用双环糊精（CD）体系，在毛细管电泳（CE）中可以成功实现酸性和中性化合物的对映体拆分。本章描述了如何使用由带负电荷的CD衍生物，即磺丁基-β-环糊精（SB-β-CD）或羧甲基-β-环糊精（CM-β-CD），与中性的七（2,3,6-三-O-甲基）-β-环糊精（TM-β-CD）组成的双CD体系来分离酸性或中性物质的对映体。选择一种酸性化合物（卡洛芬）和一种弱酸性药物（戊巴比妥）作为模型化合物。
