使用带电环糊精的非水毛细管电泳中的对映体拆分

Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins.

作者信息

Servais Anne-Catherine, Fillet Marianne

机构信息

Laboratory for the Analysis of Medicines, Department of Pharmaceutical Sciences, CIRM, Quartier Hôpital, University of Liège, Liège, Belgium.

出版信息

Methods Mol Biol. 2019;1985:373-381. doi: 10.1007/978-1-4939-9438-0_21.

DOI:10.1007/978-1-4939-9438-0_21

PMID:31069746

Abstract

The enantioseparation of acidic and basic compounds can be successfully achieved in nonaqueous capillary electrophoresis (NACE) using single-isomer charged β-cyclodextrin (β-CD) derivatives of opposite charge to that of the analytes. This chapter describes how to separate the enantiomers of three basic substances selected as model compounds, i.e., alprenolol, bupranolol, and terbutaline, using the negatively charged heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-CD (HDAS-β-CD). The enantiomers of three acidic drugs (tiaprofenic acid, suprofen, and flurbiprofen) are resolved using a monosubstituted amino β-CD derivative, namely 6-monodeoxy-6-mono(3-hydroxy)propylamino-β-CD (PA-β-CD).

摘要

使用与分析物电荷相反的单异构体带电β-环糊精（β-CD）衍生物，可在非水毛细管电泳（NACE）中成功实现酸性和碱性化合物的对映体分离。本章描述了如何使用带负电荷的七（2,3-二-O-乙酰基-6-O-磺基）-β-环糊精（HDAS-β-CD）分离三种选定为模型化合物的碱性物质，即阿普洛尔、布普洛尔和特布他林的对映体。三种酸性药物（噻洛芬酸、舒洛芬和氟比洛芬）的对映体使用单取代氨基β-环糊精衍生物，即6-单脱氧-6-单（3-羟基）丙基氨基-β-环糊精（PA-β-CD）进行拆分。