Lehrstuhl für Biochemie, Technische Universität München, Garching, Germany.
PLoS One. 2012;7(12):e52378. doi: 10.1371/journal.pone.0052378. Epub 2012 Dec 21.
The metabolic response of host cells, in particular of primary mammalian cells, to bacterial infections is poorly understood. Here, we compare the carbon metabolism of primary mouse macrophages and of established J774A.1 cells upon Listeria monocytogenes infection using (13)C-labelled glucose or glutamine as carbon tracers. The (13)C-profiles of protein-derived amino acids from labelled host cells and intracellular L. monocytogenes identified active metabolic pathways in the different cell types. In the primary cells, infection with live L. monocytogenes increased glycolytic activity and enhanced flux of pyruvate into the TCA cycle via pyruvate dehydrogenase and pyruvate carboxylase, while in J774A.1 cells the already high glycolytic and glutaminolytic activities hardly changed upon infection. The carbon metabolism of intracellular L. monocytogenes was similar in both host cells. Taken together, the data suggest that efficient listerial replication in the cytosol of the host cells mainly depends on the glycolytic activity of the hosts.
宿主细胞，特别是原代哺乳动物细胞对细菌感染的代谢反应还不甚清楚。在这里，我们使用（13）C 标记的葡萄糖或谷氨酰胺作为碳示踪剂，比较了原代小鼠巨噬细胞和已建立的 J774A.1 细胞在李斯特菌感染后的碳代谢。来自标记宿主细胞和细胞内李斯特菌的蛋白衍生氨基酸的（13）C 谱鉴定了不同细胞类型中的活跃代谢途径。在原代细胞中，活李斯特菌的感染增加了糖酵解活性，并通过丙酮酸脱氢酶和丙酮酸羧化酶增强了丙酮酸进入 TCA 循环的通量，而在 J774A.1 细胞中，感染后已有的高糖酵解和谷氨酰胺分解活性几乎没有变化。细胞内李斯特菌的碳代谢在两种宿主细胞中相似。总之，这些数据表明，在宿主细胞的细胞质中李斯特菌的有效复制主要依赖于宿主的糖酵解活性。
