Gruchlik Arkadiusz, Jurzak Magdalena, Chodurek Ewa, Dzierzewicz Zofia
Department of Biopharmacy, Medical University of Silesia, Sosnowiec, Poland.
Acta Pol Pharm. 2012 Nov-Dec;69(6):1303-6.
Cosmeceuticals represent a marriage between cosmetics and pharmaceuticals. There are numerous cosmeceutically active products which can be broadly classified into the following categories: antioxidants, oligopeptides, growth factors and pigment lightning agents. Much attention has been focused on the tripeptides such as Gly-His-Lys (GHK) and Gly-Gly-His (GGH) and their copper complexes, which have a high activity and good skin tolerance. Recent data suggested their physiological role in process of wound healing, tissue repair and skin inflammation. The mechanism of anti-inflammatory properties of these peptides is not clear. The aim of the study was evaluation of influence of two peptides GGH. GHK and their copper complexes and saccharomyces/copper ferment (Oligolides Copper) on secretion of pro-inflammatory IL-6 in normal human dermal fibroblasts NHDF cell line. IL-6 was evaluated using the ELISA kit. GGH, GHK, CuCl2 and their copper complexes decreased TNF-alpha-dependent IL-6 secretion in fibroblasts. IL-6 is crucial for normal wound healing, skin inflammation and UVB-induced erythema. Because of the anti-inflammatory properties, the copper-peptides could be used on the skin surface instead of corticosteroids or non-steroidal anti-inflammatory drugs, which have more side effects. Our observations provide some new information about the role of these tripeptides in skin inflammation.
药妆品是化妆品与药品的结合体。有许多具有药妆活性的产品，大致可分为以下几类：抗氧化剂、寡肽、生长因子和色素沉着减轻剂。人们对诸如甘氨酸-组氨酸-赖氨酸（GHK）和甘氨酸-甘氨酸-组氨酸（GGH）及其铜络合物等三肽给予了很多关注，它们具有高活性和良好的皮肤耐受性。最新数据表明它们在伤口愈合、组织修复和皮肤炎症过程中具有生理作用。这些肽的抗炎特性机制尚不清楚。本研究的目的是评估两种肽GGH、GHK及其铜络合物和酵母/铜发酵物（寡肽铜）对正常人皮肤成纤维细胞NHDF细胞系中促炎细胞因子IL-6分泌的影响。使用酶联免疫吸附测定试剂盒评估IL-6。GGH、GHK、氯化铜及其铜络合物可降低成纤维细胞中肿瘤坏死因子-α依赖性IL-6的分泌。IL-6对正常伤口愈合、皮肤炎症和紫外线B诱导的红斑至关重要。由于具有抗炎特性，铜肽可用于皮肤表面，以替代具有更多副作用的皮质类固醇或非甾体抗炎药。我们的观察结果为这些三肽在皮肤炎症中的作用提供了一些新信息。
