Gao Chenguang, Chen Hong, Niu Cong, Hu Jie, Cao Bo
a Logistics University of Chinese People's Armed Police Forces , Tianjin , China.
b Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard , Tianjin , China.
Bioengineered. 2017 Jan 2;8(1):36-44. doi: 10.1080/21655979.2016.1227572. Epub 2016 Sep 30.
Schizandrin B is extracted from Schisandra chinensis (Turcz.) Baill. This study evaluated the photoprotective effect of Schizandrin B on oxidative stress injury of the skin caused by UVB-irradiation and the molecular mechanism of the photoprotective effect of Schizandrin B, and we firstly found that Schizandrin B could block Cox-2, IL-6 and IL-18 signal pathway to protect damage of skin cells given by UVB-irradiation. In the research, we found that Schizandrin B can attenuate the UVB-induced toxicity on keratinocytes and dermal fibroblasts in human body, and can outstandingly eliminated intracellular ROS produced by UVB-irradiation. These results demonstrate that Schizandrin B can regulate the function of decreasing intracellular SOD's activity and increasing the expression level of MDA in HaCaT cells result from the guidance of UVB, and it markedly reduced the production of inflammatory factors such as Cox-2, IL-6 or IL-18, decreased the expression level of MMP-1, and interdicted degradation process of collagens in UVB-radiated cells. Therefore, skin keratinocytes can be effectively protected from UVB-radiated damage by Schizandrin B, and UVB-irradiation caused inflammatory responses can be inhibited by attenuating process of ROS generating.
五味子乙素从五味子（Schisandra chinensis (Turcz.) Baill.）中提取。本研究评估了五味子乙素对紫外线B（UVB）辐射所致皮肤氧化应激损伤的光保护作用及其光保护作用的分子机制，并且我们首次发现五味子乙素可阻断Cox-2、IL-6和IL-18信号通路，以保护UVB辐射对皮肤细胞造成的损伤。在研究中，我们发现五味子乙素可减轻UVB对人体角质形成细胞和真皮成纤维细胞的毒性作用，并且可显著清除UVB辐射产生的细胞内活性氧（ROS）。这些结果表明，UVB照射可导致HaCaT细胞内超氧化物歧化酶（SOD）活性降低、丙二醛（MDA）表达水平升高，而五味子乙素可调节其功能，并显著降低Cox-2、IL-6或IL-18等炎症因子的产生，降低基质金属蛋白酶-1（MMP-1）的表达水平，并阻断UVB辐射细胞中胶原蛋白的降解过程。因此，五味子乙素可有效保护皮肤角质形成细胞免受UVB辐射损伤，并通过减弱ROS生成过程来抑制UVB辐射引起的炎症反应。
