Gruchlik Arkadiusz, Wilczok Adam, Chodurek Ewa, Polechoński Władysław, Wolny Daniel, Dzierzewicz Zofia
Department of Biopharmacy, Medical University of Silesia, Sosnowiec, Poland.
Acta Pol Pharm. 2012 Nov-Dec;69(6):1320-4.
Intestinal subepithelial myofibroblasts play crucial role in the growth and development of the intestine. Colitis, small bowel injury, gastric ulcer disease and inflammatory bowel disease (IBD) accompany the increase of number of activated myofibroblasts. In the last few years, the increasing production of electromagnetic (EMF) and static magnetic fields (SMF), due to the expanding use of electronic devices in everyday life, has led to a number of studies on the effects of these fields on living organisms. EMF therapy, because of its anti-inflammatory properties, may be used in medicine in IBD treatment. This mechanism has not been elucidated yet. In the present work normal human colon myofibroblasts CCD-18Co were exposed to SMF with a flux density of 300 mT. After 24 h incubation TNF-alpha-dependent IL-6 secretion was determined with ELISA kit (RandD Systems).The influence of magnetic field and its effect on cell proliferation were determined with TOX-2 (In Vitro Toxicology Assay Kit XTT Based, TOX-2, Sigma) and CyQUANT NF cell proliferation assay kit (Molecular Probes). It was shown that SMF inhibited TNF-alpha-dependent IL-6 secretion. The observed effects were statistically significant and depended on the time of incubation. Moreover, SMF triggered cell proliferation whereas it did not alter cell viability. IL-6 belongs to pro-inflammatory cytokines family and plays a crucial role in IBD. Inhibition of IL-6 secretion by SMF and lack of its cytotoxic effect seem to be advantageous whilst SMF is implicated in the treatment of inflammatory diseases associated by increase in number of activated myofibroblasts.
肠道上皮下肌成纤维细胞在肠道的生长和发育中起关键作用。结肠炎、小肠损伤、胃溃疡疾病和炎症性肠病（IBD）伴随着活化肌成纤维细胞数量的增加。在过去几年中，由于日常生活中电子设备的广泛使用，电磁（EMF）和静磁场（SMF）的产生不断增加，引发了一系列关于这些场对生物体影响的研究。EMF疗法因其抗炎特性，可用于IBD治疗的医学领域。然而，这一机制尚未阐明。在本研究中，将正常人结肠肌成纤维细胞CCD-18Co暴露于通量密度为300 mT的SMF中。孵育24小时后，用ELISA试剂盒（R&D Systems）测定肿瘤坏死因子-α依赖性白细胞介素-6的分泌。用TOX-2（基于XTT的体外毒理学检测试剂盒，TOX-2，Sigma）和CyQUANT NF细胞增殖检测试剂盒（Molecular Probes）测定磁场的影响及其对细胞增殖的作用。结果表明，SMF抑制肿瘤坏死因子-α依赖性白细胞介素-6的分泌。观察到的效应具有统计学意义，且取决于孵育时间。此外，SMF可触发细胞增殖，但不改变细胞活力。白细胞介素-6属于促炎细胞因子家族，在IBD中起关键作用。SMF抑制白细胞介素-6的分泌且无细胞毒性作用，这在SMF用于治疗与活化肌成纤维细胞数量增加相关的炎症性疾病时似乎具有优势。
