吖啶橙染色与革兰氏染色在细菌性角膜炎中的比较。

Comparison of acridine orange and Gram stains in bacterial keratitis.

作者信息

Gomez J T, Robinson N M, Osato M S, Wilhelmus K R

机构信息

Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas.

出版信息

Am J Ophthalmol. 1988 Dec 15;106(6):735-7. doi: 10.1016/0002-9394(88)90710-6.

DOI:10.1016/0002-9394(88)90710-6

PMID:3195652

Abstract

We assessed the comparative sensitivities of acridine orange and Gram stains in the examination of corneal scrapings using an experimental model of Pseudomonas aeruginosa keratitis. Acridine orange was more sensitive than Gram stain, requiring concentrations of about 10(4) colony-forming units/mg of corneal tissue compared to approximately 10(5) colony-forming units/mg. Our clinical experience with 21 consecutive cases of suspected microbial keratitis showed a similar diagnostic accuracy of acridine orange and Gram stain. Acridine orange accurately predicted culture results in 15 of 21 specimens (71%) compared to a diagnostic accuracy of 62% (13 of 21 specimens) for Gram stain.

摘要

我们使用铜绿假单胞菌角膜炎实验模型，评估了吖啶橙染色和革兰氏染色在角膜刮片检查中的相对敏感性。吖啶橙染色比革兰氏染色更敏感，检测角膜组织中细菌所需的浓度约为10⁴ 菌落形成单位/毫克，而革兰氏染色约为10⁵ 菌落形成单位/毫克。我们对21例连续的疑似微生物性角膜炎病例的临床经验表明，吖啶橙染色和革兰氏染色具有相似的诊断准确性。吖啶橙染色准确预测了21个标本中15个（71%）的培养结果，而革兰氏染色的诊断准确率为62%（21个标本中的13个）。