Liu Cheng-Bo, Song Jing-Bo, Long Tao, Zhang Zhen-Xi
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2012 Oct;32(10):2714-7.
Steady-state spectroscopy is prevailing for complementary tissue structural and functional information extraction The goal of the present study is to experimentally validate the merit of this technique for optical properties and intrinsic fluorescence extraction from tissue mimicking phantoms. Phantom studies were carried out in which HbO2 was used as absorber, polystyrene sphere was used as scatterer, and stilbene was used for fluorescence emission Two sets of phantoms corresponding to two absorption levels and one scattering level were made, and 4 fluorophore concentrations were titrated in each set. The results indicate that both optical properties and intrinsic fluorescence can be extracted with high accuracy, implying that it's feasible to extract complementary structural and functional information with steady-state spectroscopy.
稳态光谱法在提取互补的组织结构和功能信息方面很常用。本研究的目的是通过实验验证该技术从仿组织体模中提取光学特性和固有荧光的优点。进行了体模研究，其中使用 HbO2 作为吸收体，聚苯乙烯球体作为散射体，芪用于荧光发射。制作了两组对应于两种吸收水平和一种散射水平的体模，每组中滴定了 4 种荧光团浓度。结果表明，光学特性和固有荧光都可以高精度提取，这意味着用稳态光谱法提取互补的结构和功能信息是可行的。
