[从经乙二胺四乙酸（EDTA）处理的质膜上清液中纯化一种可改变钠钾ATP酶对哇巴因敏感性的膜蛋白]
[Purification of a membrane protein modifying the sensitivity of Na+/K+ ATPase to ouabain from a supernatant of plasma membrane treated with EDTA].
作者信息
Geny B, Charlemagne D, Paraf A
出版信息
C R Seances Acad Sci D. 1979 Nov 26;289(14):1057-60.
PMID:232860
Abstract
A membrane protein of M. W. 32,000 D and pI 5 has been purified from EDTA-treated plasma membrane supernatants by Page without detergents. These membranes are purified from the MF2S cell line issued from the murine plasmacytoma MOPC 173. The purified protein was able to induce a 300 fold increase in the Na+/K+ ATPase resistance to ouabaïn thus mimicking the original resistance of the enzyme to the drug.
摘要
一种分子量为32000道尔顿、等电点为5的膜蛋白已通过无去污剂的聚丙烯酰胺凝胶电泳从经乙二胺四乙酸（EDTA）处理的质膜上清液中纯化出来。这些膜是从源自小鼠浆细胞瘤MOPC 173的MF2S细胞系中纯化得到的。纯化后的蛋白能够使钠钾ATP酶对哇巴因的抗性提高300倍，从而模拟了该酶对药物的原始抗性。
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