[从一种可改变钠钾ATP酶对哇巴因敏感性的蛋白质的全细胞裂解物中进行纯化]
[Purification from a total cellular lysate of a protein modifying the sensitivity of Na+/K+ ATPase to ouabain].
作者信息
Charlemagne D, Geny B, Paraf A
出版信息
C R Seances Acad Sci D. 1979 Dec 10;289(15):1137-40.
PMID:232861
Abstract
A protein (32,000 dalton) has been purified from M2FS cells derived from the murine plasmocytoma MOPC 173. Like tropomyosin, this protein when added with Ca++ to EDTA-treated plasma membranes prepared from the same cell, induced a drastic increase in the Na+/K+ atpase resistance to ouabain.
摘要
一种蛋白质（32,000道尔顿）已从源自鼠浆细胞瘤MOPC 173的M2FS细胞中纯化出来。与原肌球蛋白一样，这种蛋白质在与钙离子一起添加到由同一细胞制备的经乙二胺四乙酸（EDTA）处理的质膜时，会使钠钾ATP酶对哇巴因的抗性急剧增加。
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