Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA.
Exp Eye Res. 2013 Mar;108:72-5. doi: 10.1016/j.exer.2012.11.022. Epub 2012 Dec 31.
The purpose of the present study was to investigate whether systemically administered resveratrol can protect against acute retinal ischemic reperfusion injury. Two groups of adult male Sprague Dawley rats (n = 6 per group) were used for this study. Resveratrol (30 mg/kg) or an equal volume of vehicle (30% Solutol HS 15 in 0.9% saline) was administered daily for 5 days via intraperitoneal injection. On the third day of treatment, retinal ischemic injury was induced by elevation of intraocular pressure for 45 min. Prior to resveratrol administration and one-week following ischemic insult, retinal function was measured by scotopic electroretinography (ERG). Retinas were harvested and morphologically analyzed one week after ischemic insult. ERG a- and b-wave amplitudes were significantly reduced following ischemic reperfusion injury. Resveratrol treatment attenuated ischemic-induced loss of retinal function. In control vehicle-treated rats, ischemic reperfusion injury elicited marked thinning of inner retinal layers. Resveratrol prophylactic treatment reduced ischemia-mediated thinning of the whole retina and in particular the inner retinal layers. Therefore, resveratrol may have therapeutic value for the management of retinal ischemic disorders.
本研究旨在探讨系统给予白藜芦醇是否可以预防急性视网膜缺血再灌注损伤。本研究使用了两组成年雄性 Sprague Dawley 大鼠（每组 n=6）。白藜芦醇（30mg/kg）或等体积的载体（30% Solutol HS 15 在 0.9%生理盐水中）通过腹腔注射每天给药 5 天。在治疗的第三天，通过升高眼内压诱导视网膜缺血 45 分钟。在白藜芦醇给药之前和缺血损伤后一周，通过暗视视网膜电图（ERG）测量视网膜功能。在缺血损伤后一周收获视网膜并进行形态学分析。缺血再灌注损伤后 ERG a-和 b-波幅度明显降低。白藜芦醇治疗减轻了缺血引起的视网膜功能丧失。在对照载体处理的大鼠中，缺血再灌注损伤引起内视网膜层明显变薄。白藜芦醇预防性治疗减少了缺血介导的整个视网膜变薄，特别是内视网膜层。因此，白藜芦醇可能对视网膜缺血性疾病的治疗具有重要价值。
