缺氧状态下艾氏腹水癌细胞中组蛋白H1(0)信使核糖核酸水平升高。

Increased level of histone H1(0) messenger RNA in hypoxic Ehrlich ascites tumor cells.

作者信息

Bouterfa H, Doenecke D, Löffler M

机构信息

Institute of Biochemistry, University of Göttingen, Federal Republic of Germany.

出版信息

Exp Cell Res. 1990 May;188(1):160-3. doi: 10.1016/0014-4827(90)90292-i.

DOI:10.1016/0014-4827(90)90292-i

PMID:2328774

Abstract

We have investigated the expression of the H1 histone subtype H1(0) gene in Ehrlich ascites tumor cells (EAT) under varied conditions of oxygen supply. Our results show that proliferating EAT cells express H1(0) mRNA at a basal level under normoxic conditions. Severe hypoxia leads to a cessation of cell growth and causes an accumulation of cells in G1. Here, we show that the level of H1(0) histone mRNA increases within a few hours after the onset of hypoxia.

摘要

我们研究了在不同氧气供应条件下，艾氏腹水瘤细胞（EAT）中H1组蛋白亚型H1(0)基因的表达情况。我们的结果表明，在常氧条件下，增殖的EAT细胞以基础水平表达H1(0) mRNA。严重缺氧导致细胞生长停止，并使细胞在G1期积累。在此，我们表明缺氧开始后数小时内，H1(0)组蛋白mRNA水平升高。