IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Eur J Clin Microbiol Infect Dis. 2013 Jun;32(6):781-6. doi: 10.1007/s10096-012-1808-2. Epub 2013 Jan 4.
We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
我们开发了一种使用新型肽核酸 (PNA) 探针的荧光原位杂交 (FISH) 方法，用于快速检测尿液中的变形菌属。对 137 份尿路感染患者的尿液样本进行检测，与 CHROMagar Orientation 培养基相比，该方法的特异性和灵敏度分别为 98%（95%CI，93.2-99.7）和 100%（95%CI，80.8-100）。结果表明，PNA-FISH 是传统培养方法的可靠替代方法，可将诊断时间缩短至约 2 小时。
