Kumar Shashank, Pandey Sanjay, Pandey Abhay K
Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Instrumentation Division, Indian Institute of Integrative Medicine, Jammu 180001, India.
Biomed Res Int. 2014;2014:495154. doi: 10.1155/2014/495154. Epub 2014 May 7.
Present work reports the biological activities of P. hysterophorus leaf, stem, flower, and root. Dried samples were sequentially extracted with many solvents. Hexane (HX), benzene (BZ), and chloroform (CH) extracts of leaf showed considerable antibacterial activity against Streptococcus mutans (MTCC 497), Proteus vulgaris (MTCC 7299), and Salmonella typhi (MTCC 3917). Flower extracts exhibited presence of higher amount of flavonoids (13.9-59.6 μgQE/mg) followed by leaf, stem, and root. Stem (HX, BZ, and CH), leaf ethanol (ET), and root (HX, BZ, and CH) fractions showed noticeable antioxidant capacity in phosphomolybdate assay. Most of the extracts demonstrated beta carotene bleaching inhibition capability. BZ, ethyl acetate (EA), and ET fractions of leaves, stem aqueous (AQ), and flower EA extracts showed membrane protective activities (40-55%). Middle fractions of the plant parts displayed moderate antihemolytic potential. Most of the flower extracts exhibited cytotoxic activity (80-100%) against lung and colon cancer cell lines. Root (HX and ET) and leaf ET extracts showed considerable inhibition (90-99%) of colon and ovary cancer cell lines. The LC-MS scan demonstrated presence of different compounds showing 3-20 min retention time. The study revealed considerable antibacterial, antioxidant, lipo-protective, antihemolytic, and anticancer potential in all parts of P. hysterophorus.
当前的研究报告了假苍耳叶、茎、花和根的生物活性。将干燥的样品依次用多种溶剂进行提取。叶的己烷（HX）、苯（BZ）和氯仿（CH）提取物对变形链球菌（MTCC 497）、普通变形杆菌（MTCC 7299）和伤寒沙门氏菌（MTCC 3917）显示出显著的抗菌活性。花提取物中黄酮类化合物含量较高（13.9 - 59.6 μgQE/mg），其次是叶、茎和根。茎（HX、BZ和CH）、叶乙醇提取物（ET）和根（HX、BZ和CH）部分在磷钼酸盐测定中显示出显著的抗氧化能力。大多数提取物表现出β - 胡萝卜素漂白抑制能力。叶的BZ、乙酸乙酯（EA）和ET部分、茎水提取物（AQ）和花EA提取物显示出膜保护活性（40 - 55%）。植物各部分的中间部分显示出适度的抗溶血潜力。大多数花提取物对肺癌和结肠癌细胞系表现出细胞毒性活性（80 - 100%）。根（HX和ET）和叶ET提取物对结肠和卵巢癌细胞系显示出显著的抑制作用（90 - 99%）。LC - MS扫描显示存在保留时间为3 - 20分钟的不同化合物。该研究揭示了假苍耳所有部分都具有显著的抗菌、抗氧化、脂质保护、抗溶血和抗癌潜力。
