Terabe S, Konaka R, Inouye K
J Chromatogr. 1979 Apr 21;172:163-77. doi: 10.1016/s0021-9673(00)90954-3.
Twenty-one analogues of ACTH, three analogues of LH-RH and four insulins have been successfully separated on a commercial reversed-phase material with tartrate buffer--acetonitrile systems containing sodium 1-butanesulphonate and sodium sulphate as the mobile phase. The effect of the constituent amino acid residues on the order of elution has been studied in detail by using a variety of closely related peptides; the order of elution of a series of peptides, which differ by only one amino acid residue, can in most instances be explained in terms of the difference in the hydrophobicities of the amino acid residues concerned, but in some instances, such as in diastereoisomers or positional isomers, the order of elution must be interpreted in terms of the hydrophobicity of the whole peptide molecule. This chromatographic method has been proved to be very useful for the rapid examination of the purity of these peptide hormones and for the separation of closely related peptides with molecular weights up to ca. 6000.
在以含有1-丁烷磺酸钠和硫酸钠的酒石酸盐缓冲液 - 乙腈体系作为流动相的商用反相材料上，已成功分离出21种促肾上腺皮质激素类似物、3种促黄体生成素释放激素类似物和4种胰岛素。通过使用多种密切相关的肽，详细研究了组成氨基酸残基对洗脱顺序的影响；一系列仅相差一个氨基酸残基的肽的洗脱顺序，在大多数情况下可以根据相关氨基酸残基疏水性的差异来解释，但在某些情况下，如非对映异构体或位置异构体中，洗脱顺序必须根据整个肽分子的疏水性来解释。事实证明，这种色谱方法对于快速检测这些肽类激素的纯度以及分离分子量高达约6000的密切相关肽非常有用。
