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Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.

作者信息

Hancock W S, Bishop C A, Prestidge R L, Harding D R, Hearn M T

出版信息

Science. 1978 Jun 9;200(4346):1168-70. doi: 10.1126/science.206966.

DOI:10.1126/science.206966
PMID:206966
Abstract

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).

摘要

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Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.
Science. 1978 Jun 9;200(4346):1168-70. doi: 10.1126/science.206966.
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