[交联剂对复合生物海绵的影响]

[The influences of cross-linking agent on the composite bio-sponge].

作者信息

Li Donghong, Li Pengxi, Luo Juxiu, Huang Lu

机构信息

State key laboratory of Trauma, Burn and Combined Injury, the 2nd Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.

出版信息

Zhongguo Yi Liao Qi Xie Za Zhi. 2012 Sep;36(5):317-20.

PMID:23289331

Abstract

OBJECTIVE

To investigate the influence of cross-linking agents genipin and glutaraldehyde on the composite bio-sponge.

METHODS

The composite bio-sponge was prepared with the technology of lyophilization. The degree of cross linking was determined using absorptiometry of trinitrobenzenesulfonic acid; the cytotoxicity was tested by MTT assay; the degradation rate in vitro was valuated by lysozyme degradation.

RESULTS

(1) The degree of cross linking of composite bio-sponge crosslinked using genipin and glutaraldehyde increased with the crosslinking time, and reached 26.43% and 54.63% respectively after crosslinking 3 d. (2) The water absorption rate of composite bio-sponge crosslinked using genipin was better than that of crosslinked using glutaraldehyde. (3) In the initial stage of cells incubation, all extracts of composite bio-sponges crosslinked using genipin and glutaraldehyde inhibited the growth of the cells, and the inhibition decreased with the incubation time; but the cytotoxicity of composite bio-sponge crosslinked using glutaraldehyde was higher than that of crosslinked using genipin. (4) After soaking in saline for 4 weeks, the degradation rate of composite bio-sponge crosslinked using genipin or glutaraldehyde was 32.1%, 28.4%, respectively; however, after soaking in saline containing lysozyme for 40 h, the degradation rate of composite bio-sponge was 36.7%, 31.2%, respectively.

CONCLUSION

Compared with the composite bio-sponge crosslinked using glutaraldehyde, the degree of cross linking and the cytotoxicity of the composite bio-sponge crosslinked using genipin decreased; however, the water absorption rate and the degradation rate increased.

摘要

目的

探讨交联剂京尼平和戊二醛对复合生物海绵的影响。

方法

采用冻干技术制备复合生物海绵。用三硝基苯磺酸吸光光度法测定交联度；用MTT法检测细胞毒性；通过溶菌酶降解评估体外降解率。

结果

（1）用京尼平和戊二醛交联的复合生物海绵的交联度随交联时间增加，交联3 d后分别达到26.43%和54.63%。（2）用京尼平交联的复合生物海绵的吸水率优于用戊二醛交联的。（3）在细胞培养初期，用京尼平和戊二醛交联的复合生物海绵的所有提取物均抑制细胞生长，且抑制作用随培养时间降低；但用戊二醛交联的复合生物海绵的细胞毒性高于用京尼平交联的。（4）在盐水中浸泡4周后，用京尼平或戊二醛交联的复合生物海绵的降解率分别为32.1%、28.4%；然而，在含溶菌酶的盐水中浸泡40 h后，复合生物海绵的降解率分别为36.7%、31.2%。