Herbal and Indian Medicine Research Laboratory, Department of Biochemistry, Sri Ramachandra University, Chennai, India.
Phytomedicine. 2013 Feb 15;20(3-4):246-8. doi: 10.1016/j.phymed.2012.11.006. Epub 2013 Jan 3.
Tinospora cordifolia is a well reported plant possessing numerous medicinal values including anti-diabetic property. Aim of the present study is to study the mechanism of action of Tinospora cordifolia and its active compound in differentiated myocytes, L6 cells. Key marker of diabetes in cells is the insulin dependent glucose transporter-4 (Glut-4) which also responds to exogenous chemicals, and is over expressed up to 5- and 4-fold, by Tinospora cordifolia and palmatine, respectively. Next to Glut-4, the predominant protein influencing glucose metabolism is PPARα and γ whose expressions were also positively modulated. Further, the inhibitors of insulin pathway prevented glucose uptake mediated by Tinospora cordifolia and palmatine which shows that the activity is majorly mediated through insulin pathway.
三叶鬼针草是一种具有多种药用价值的植物，包括抗糖尿病特性。本研究的目的是研究三叶鬼针草及其活性化合物在分化的肌细胞（L6 细胞）中的作用机制。细胞中糖尿病的关键标志物是胰岛素依赖性葡萄糖转运蛋白-4（Glut-4），它也对外源化学物质有反应，并且分别被三叶鬼针草和小檗碱上调表达 5 倍和 4 倍。除了 Glut-4，影响葡萄糖代谢的主要蛋白质是 PPARα 和 γ，它们的表达也被正向调节。此外，胰岛素途径的抑制剂可阻止三叶鬼针草和小檗碱介导的葡萄糖摄取，这表明该活性主要通过胰岛素途径介导。
