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本文引用的文献

1
Dynamics in cryo EM reconstructions visualized with maximum-likelihood derived variance maps.利用最大似然法推导的方差图可视化低温电子显微镜重构中的动态变化。
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Nucleotide-resolution profiling of RNA recombination in the encapsidated genome of a eukaryotic RNA virus by next-generation sequencing.通过下一代测序对真核 RNA 病毒衣壳基因组中 RNA 重组进行核苷酸分辨率分析。
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Movies of ice-embedded particles enhance resolution in electron cryo-microscopy.冰嵌入颗粒的电影提高了电子冷冻显微镜的分辨率。
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Packaging host RNAs in small RNA viruses: an inevitable consequence of an error-prone polymerase?小RNA病毒中包装宿主RNA：易出错的聚合酶的必然结果？
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Maturation in action: CryoEM study of a viral capsid caught during expansion.在行动中成熟：冷冻电镜研究在扩张过程中捕获的病毒衣壳。
Structure. 2012 Aug 8;20(8):1384-90. doi: 10.1016/j.str.2012.05.011. Epub 2012 Jun 28.
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Host RNAs, including transposons, are encapsidated by a eukaryotic single-stranded RNA virus.真核单链 RNA 病毒将宿主 RNA（包括转座子）包裹起来。
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Mechanics of bacteriophage maturation.噬菌体成熟的机制。
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8
Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy.直接检测设备探测器用于单颗粒冷冻电子显微镜的初步评估。
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The Prohead-I structure of bacteriophage HK97: implications for scaffold-mediated control of particle assembly and maturation.噬菌体 HK97 的 Prohead-I 结构：对支架介导的颗粒组装和成熟控制的影响。
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一位二十面体病毒晶体学家的自白。

Confessions of an icosahedral virus crystallographer.

作者信息

Johnson John E

机构信息

Department of Molecular Biology, MB31, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.

出版信息

Microscopy (Oxf). 2013 Feb;62(1):69-79. doi: 10.1093/jmicro/dfs097. Epub 2013 Jan 4.

DOI:10.1093/jmicro/dfs097
PMID:23291268
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3624677/
Abstract

This is a personal history of my structural studies of icosahedral viruses that evolved from crystallographic studies, to hybrid methods with electron cryo-microscopy and image reconstruction (cryoEM) and then developed further by incorporating a variety of physical methods to augment the high resolution crystallographic studies. It is not meant to be comprehensive, even for my own work, but hopefully provides some perspective on the growth of our understanding of these remarkable biologic assemblies. The goal is to provide a historical perspective for those new to the field and to emphasize the limitations of any one method, even those that provide atomic resolution information about viruses.

摘要

这是我对二十面体病毒结构研究的个人历程，该研究从晶体学研究起步，发展到结合电子冷冻显微镜和图像重建（冷冻电镜）的混合方法，之后通过纳入多种物理方法进一步拓展，以强化高分辨率晶体学研究。即便对于我自己的工作而言，本文也无意做到全面，而是希望能为我们对这些非凡生物聚集体的理解发展提供一些视角。目标是为该领域的新手提供一个历史视角，并强调任何一种方法的局限性，即使是那些能提供病毒原子分辨率信息的方法。