一位二十面体病毒晶体学家的自白。
Confessions of an icosahedral virus crystallographer.
作者信息
Johnson John E
机构信息
Department of Molecular Biology, MB31, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Microscopy (Oxf). 2013 Feb;62(1):69-79. doi: 10.1093/jmicro/dfs097. Epub 2013 Jan 4.
Abstract
This is a personal history of my structural studies of icosahedral viruses that evolved from crystallographic studies, to hybrid methods with electron cryo-microscopy and image reconstruction (cryoEM) and then developed further by incorporating a variety of physical methods to augment the high resolution crystallographic studies. It is not meant to be comprehensive, even for my own work, but hopefully provides some perspective on the growth of our understanding of these remarkable biologic assemblies. The goal is to provide a historical perspective for those new to the field and to emphasize the limitations of any one method, even those that provide atomic resolution information about viruses.
摘要
这是我对二十面体病毒结构研究的个人历程，该研究从晶体学研究起步，发展到结合电子冷冻显微镜和图像重建（冷冻电镜）的混合方法，之后通过纳入多种物理方法进一步拓展，以强化高分辨率晶体学研究。即便对于我自己的工作而言，本文也无意做到全面，而是希望能为我们对这些非凡生物聚集体的理解发展提供一些视角。目标是为该领域的新手提供一个历史视角，并强调任何一种方法的局限性，即使是那些能提供病毒原子分辨率信息的方法。
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