Cai Xin, Yang Yong-chang, Wang Jing-feng, Wang Qiang, Gao Jie, Fu Wen-liang, Zhu Ze-yi, Wang Yuan-yuan, Zou Min-ji, Wang Jia-xi, Xu Dong-qun, Xu Dong-gang
Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, China.
J Asthma. 2013 Mar;50(2):209-14. doi: 10.3109/02770903.2012.753454. Epub 2013 Jan 7.
The presence of transient receptor potential vanilloid 2 (TRPV2) in human peripheral blood cells may suggest a role under pathological conditions. The aim of this study was to explore the relationship between the expression profile of TRPV2 gene and childhood asthma in the north of China. The effects of allergens exposure on the expression of TRPV2 gene were also investigated.
Sixty asthmatics children confirmed by physician diagnosis and 60 healthy children as a control group were recruited. Serum total IgE and specific IgE were measured. Using quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR), TRPV2 was detected in total RNA extracted from peripheral blood lymphocytes. Student's t-test and chi-square test were used to analyze the relationship between TRPV2 transcript and different parameter variables on susceptibility of childhood asthma. Multiple logistic regression was used to analyze the associations between TRPV2 gene and allergens.
The expression level of TRPV2 gene was increased 2.6 times in asthmatic children compared with controls (p < .01). The up-regulation of TRPV2 gene and sensitization to one of three the allergens-spring pollen, dust mite, and dog and cat hair-were correlated with childhood asthma. In addition, the hypersensitivity to spring pollen, cockroach, and dust mite and up-regulation of TRPV2 gene expression may be the risk factors for the childhood asthma in Beijing.
The increased expression of TRPV2 gene in peripheral lymphocytes is closely correlated with childhood asthma in the north of China. This study provides a potential new biomarker of childhood asthma and lays the basis for further clarification of the pathogenesis underlying asthma.
人类外周血细胞中存在瞬时受体电位香草酸亚型2（TRPV2），这可能提示其在病理条件下起一定作用。本研究旨在探讨中国北方TRPV2基因表达谱与儿童哮喘之间的关系。同时还研究了过敏原暴露对TRPV2基因表达的影响。
招募60例经医生诊断确诊的哮喘儿童作为病例组，60例健康儿童作为对照组。检测血清总IgE和特异性IgE。采用定量逆转录聚合酶链反应（qRT-PCR）检测外周血淋巴细胞提取的总RNA中的TRPV2。采用Student's t检验和卡方检验分析TRPV2转录本与儿童哮喘易感性不同参数变量之间的关系。采用多因素logistic回归分析TRPV2基因与过敏原之间的关联。
与对照组相比，哮喘儿童TRPV2基因表达水平升高了2.6倍（p <.01）。TRPV2基因上调以及对三种过敏原之一（春季花粉、尘螨、猫狗毛发）致敏与儿童哮喘相关。此外，对春季花粉、蟑螂和尘螨过敏以及TRPV2基因表达上调可能是北京地区儿童哮喘的危险因素。
外周淋巴细胞中TRPV2基因表达增加与中国北方儿童哮喘密切相关。本研究为儿童哮喘提供了一个潜在的新生物标志物，并为进一步阐明哮喘发病机制奠定了基础。
