Miller D C, Peron S E, Keck R W, Kropp K A
Division of Urology, Medical College of Ohio, Toledo 43699.
J Urol. 1990 May;143(5):1046-8. doi: 10.1016/s0022-5347(17)40180-7.
The ischemic effects of prolonged testicular torsion have been well documented; however, prevention or arrest of the damaging effects of prolonged ischemia has been incompletely studied. Two groups of Sprague-Dawley rats were subjected to varying lengths of bilateral testicular ischemia. Group I underwent normothermic ischemia for two, four, and six hours. Likewise, Group II underwent similar time periods of ischemia, however, after thirty minutes of normothermic ischemia the scrotum of each animal was placed into an ice bath maintained at 4C. Two weeks postoperatively, bilateral orchiectomy was performed. Histology of the testes of the two groups was compared. Neither group revealed significant destruction of the germinal epithelium after two hours of ischemia. Group I revealed only 25% preservation of the germinal epithelium at four hours and only 8% preservation at six hours of ischemia. In contrast, Group II which received ice showed 90% preservation of germinal epithelium at four hours and 85% preservation at six hours of ischemia. We conclude that external ice application significantly preserves seminiferous tubules at four and six hours of ischemic injury in the rat testicle.
长时间睾丸扭转的缺血效应已有充分记录；然而，对于预防或阻止长时间缺血的损伤效应的研究并不完整。两组斯普拉格-道利大鼠经历了不同时长的双侧睾丸缺血。第一组进行了2小时、4小时和6小时的常温缺血。同样，第二组也经历了相似时长的缺血，不过，在常温缺血30分钟后，将每只动物的阴囊放入维持在4℃的冰浴中。术后两周，进行双侧睾丸切除术。比较两组睾丸的组织学情况。两组在缺血2小时后均未显示出生殖上皮的显著破坏。第一组在缺血4小时时仅显示25%的生殖上皮保留，在缺血6小时时仅保留8%。相比之下，接受冰敷的第二组在缺血4小时时显示90%的生殖上皮保留，在缺血6小时时保留85%。我们得出结论，在大鼠睾丸缺血损伤4小时和6小时时，外部冰敷能显著保护生精小管。
