Department of Radiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Neuroimage. 2013 May 15;72:1-9. doi: 10.1016/j.neuroimage.2012.12.056. Epub 2013 Jan 5.
Pharmacological magnetic resonance imaging (phMRI) maps the neurovascular response to a pharmacological challenge and is increasingly used to assess neurotransmitter systems. Here we investigated the hemodynamic response to a dopaminergic (DAergic) challenge with dextroamphetamine (dAMPH) in humans using arterial spin labeling (ASL) based phMRI. Twelve healthy male subjects aged 21.0years (±1.5) were included. We used a pseudo-continuous ASL sequence (40min) to quantify cerebral blood flow (CBF) and started dAMPH infusion (0.3mg/kg) after 10min. On another day, we measured baseline dopamine D2/3 receptor availability with [(123)I]IBZM single photon emission computed tomography (SPECT). Baseline measures on mood and impulsivity and subjective behavioral responses to dAMPH were obtained. CBF response was corrected for cardiovascular effects using an occipital cortex mask for internal reference. Corrected CBF (sCBF) was analyzed using ROI-based and voxel-based analysis, in addition to independent component analysis (ICA). CBF data was correlated to D2/3 receptor availability and behavioral measures. Subjects reported experiencing euphoria following dAMPH administration. In the striatum sCBF significantly increased, as demonstrated by all three analysis methods. Voxel-based analysis and ICA also showed increased sCBF in the thalamus, anterior cingulate and cerebellum. Decreased sCBF was observed in several cortical areas, the posterior cingulated and paracingulate cortex. Apart from one ICA component, no correlations were found with sCBF changes and D2/3 receptor availability and behavioral measures. Our observations are in line with literature and provide further evidence that ASL-based phMRI with dAMPH is a promising technique to assess DAergic function in human subjects.
药理学磁共振成像（phMRI）可绘制神经血管对药理学挑战的反应图，并且越来越多地用于评估神经递质系统。在这里，我们使用基于动脉自旋标记（ASL）的 phMRI 研究了人体对多巴胺能（DAergic）挑战的血液动力学反应，使用了右旋苯丙胺（dAMPH）。纳入了 12 名年龄为 21.0 岁（±1.5）的健康男性受试者。我们使用伪连续 ASL 序列（40min）来量化脑血流（CBF），并在 10min 后开始 dAMPH 输注（0.3mg/kg）。在另一天，我们使用[123I]IBZM 单光子发射计算机断层扫描（SPECT）测量了基线多巴胺 D2/3 受体可及性。获得了关于情绪和冲动的基线测量以及对 dAMPH 的主观行为反应。使用枕叶皮质掩模作为内部参考，校正了心血管效应后的 CBF 反应。使用 ROI 基于和体素基于的分析以及独立成分分析（ICA）来分析校正后的 CBF（sCBF）。CBF 数据与 D2/3 受体可及性和行为测量相关。受试者报告在 dAMPH 给药后出现欣快感。所有三种分析方法均表明，纹状体中的 sCBF 显着增加。基于体素的分析和 ICA 还显示丘脑、前扣带和小脑中的 sCBF 增加。在几个皮质区域、后扣带和旁扣带皮层观察到 sCBF 减少。除了一个 ICA 分量外，sCBF 变化与 D2/3 受体可及性和行为测量之间没有发现相关性。我们的观察结果与文献一致，并进一步证明基于 ASL 的 phMRI 与 dAMPH 是评估人类受试者 DAergic 功能的有前途的技术。
