Matsushita Kohei, Uchida Keiichi, Saigusa Susumu, Ide Shozo, Hashimoto Kiyoshi, Koike Yuhki, Otake Kohei, Inoue Mikihiro, Tanaka Koji, Kusunoki Masato
Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, Japan.
Pediatr Surg Int. 2013 Apr;29(4):363-8. doi: 10.1007/s00383-012-3248-6.
N-myc downstream regulated gene 1 (NDRG1) markedly reduces metastasis of numerous tumors. However, NDRG1's function in malignant tumors has not been fully determined. Therefore, we investigated the association of NDRG1 expression with clinical outcomes in neuroblastoma (NB) patients.
We obtained total RNA from residual cancer cells using microdissection from NB patients. Furthermore, we examined the expression of NDRG1 in NB patients using immunohistochemical staining.
Of the 48 patients observed, low NDRG1 expression was associated with poor prognostic factors such as primary tumor size and MYCN amplification. Low expression of NDRG1 was associated with a poor prognosis (p = 0.001) and multivariate analysis identified low expression of NDRG1 as an independent risk factor for predicting poor prognosis in NB patients. Furthermore, in the MYCN non-amplification group (n = 33), low expression of NDRG1 was associated with a poor prognosis (p = 0.001). Immunohistochemical analysis showed NDRG1 expression at the plasma membranes of NB cells. NDRG1 expression levels were also correlated with expression of NDRG1 mRNA.
We confirmed that low NDRG1 expression is a significant and independent prognostic indicator in NB by multivariate analysis. Furthermore, NDRG1 may be a novel prognostic marker in MYCN non-amplification NB patients.
N - myc下游调控基因1（NDRG1）可显著降低多种肿瘤的转移。然而，NDRG1在恶性肿瘤中的功能尚未完全明确。因此，我们研究了NDRG1表达与神经母细胞瘤（NB）患者临床预后的相关性。
我们通过对NB患者的癌细胞进行显微切割获取总RNA。此外，我们采用免疫组织化学染色检测NB患者中NDRG1的表达。
在观察的48例患者中，NDRG1低表达与原发肿瘤大小和MYCN扩增等不良预后因素相关。NDRG1低表达与预后不良相关（p = 0.001），多因素分析确定NDRG1低表达是预测NB患者预后不良的独立危险因素。此外，在MYCN非扩增组（n = 33）中，NDRG1低表达与预后不良相关（p = 0.001）。免疫组织化学分析显示NB细胞膜上有NDRG1表达。NDRG1表达水平也与NDRG1 mRNA表达相关。
通过多因素分析，我们证实NDRG1低表达是NB患者中一个显著且独立的预后指标。此外，NDRG1可能是MYCN非扩增NB患者的一种新型预后标志物。
