Orlando Ron
Department of Biochemistry, University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2013;951:197-215. doi: 10.1007/978-1-62703-146-2_13.
The ability to quantitatively determine changes in the N- and O-linked glycans is an essential component of comparative glycomics. Multiple strategies are available to by which this can be accomplished, including; both label free approaches and isotopic labeling strategies. The focus of this chapter is to describe each of these approaches while providing insight into their strengths and weaknesses, so that glycomic investigators can make an educated choice of the strategy that is best suited for their particular application.
定量测定N-连接和O-连接聚糖变化的能力是比较糖组学的重要组成部分。有多种策略可用于实现这一目标，包括无标记方法和同位素标记策略。本章的重点是描述每种方法，同时深入探讨其优缺点，以便糖组学研究人员能够明智地选择最适合其特定应用的策略。
