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基于定量糖组学发现上皮性卵巢癌中特定的转移相关N-聚糖改变

Discovery of specific metastasis-related N-glycan alterations in epithelial ovarian cancer based on quantitative glycomics.

作者信息

Zhang Xingwang, Wang Yisheng, Qian Yifan, Wu Xin, Zhang Zejian, Liu Xijun, Zhao Ran, Zhou Lei, Ruan Yuanyuan, Xu Jiejie, Liu Haiou, Ren Shifang, Xu Congjian, Gu Jianxin

机构信息

Key Laboratory of Glycoconjugate Research Ministry of Public Health, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.

出版信息

PLoS One. 2014 Feb 6;9(2):e87978. doi: 10.1371/journal.pone.0087978. eCollection 2014.

DOI:10.1371/journal.pone.0087978
PMID:24516574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3916363/
Abstract

Generally, most of ovarian cancer cannot be detected until large scale and remote metastasis occurs, which is the major cause of high mortality in ovarian cancer. Therefore, it is urgent to discover metastasis-related biomarkers for the detection of ovarian cancer in its occult metastasis stage. Altered glycosylation is a universal feature of malignancy and certain types of glycan structures are well-known markers for tumor progressions. Thus, this study aimed to reveal specific changes of N-glycans in the secretome of the metastatic ovarian cancer. We employed a quantitative glycomics approach based on metabolic stable isotope labeling to compare the differential N-glycosylation of secretome between an ovarian cancer cell line SKOV3 and its high metastatic derivative SKOV3-ip. Intriguingly, among total 17 N-glycans identified, the N-glycans with bisecting GlcNAc were all significantly decreased in SKOV3-ip in comparison to SKOV3. This alteration in bisecting GlcNAc glycoforms as well as its corresponding association with ovarian cancer metastatic behavior was further validated at the glycotransferase level with multiple techniques including real-time PCR, western blotting, transwell assay, lectin blotting and immunohistochemistry analysis. This study illustrated metastasis-related N-glycan alterations in ovarian cancer secretome in vitro for the first time, which is a valuable source for biomarker discovery as well. Moreover, N-glycans with bisecting GlcNAc shed light on the detection of ovarian cancer in early peritoneal metastasis stage which may accordingly improve the prognosis of ovarian cancer patients.

摘要

一般来说，大多数卵巢癌在发生大规模远处转移之前无法被检测到，这是卵巢癌死亡率高的主要原因。因此，迫切需要发现与转移相关的生物标志物，用于检测处于隐匿转移阶段的卵巢癌。糖基化改变是恶性肿瘤的一个普遍特征，某些类型的聚糖结构是肿瘤进展的著名标志物。因此，本研究旨在揭示转移性卵巢癌分泌组中N-聚糖的特定变化。我们采用基于代谢稳定同位素标记的定量糖组学方法，比较卵巢癌细胞系SKOV3及其高转移衍生物SKOV3-ip分泌组的差异N-糖基化。有趣的是，在总共鉴定出的17种N-聚糖中，与SKOV3相比，带有平分型N-乙酰葡糖胺的N-聚糖在SKOV3-ip中均显著减少。通过实时PCR、蛋白质印迹、Transwell实验、凝集素印迹和免疫组织化学分析等多种技术，在糖基转移酶水平进一步验证了平分型N-乙酰葡糖胺糖型的这种改变及其与卵巢癌转移行为的相应关联。本研究首次阐明了体外卵巢癌分泌组中与转移相关的N-聚糖改变，这也是发现生物标志物的宝贵来源。此外，带有平分型N-乙酰葡糖胺的N-聚糖为早期腹膜转移阶段卵巢癌的检测提供了线索，这可能相应地改善卵巢癌患者的预后。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8318/3916363/f7b49678b883/pone.0087978.g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8318/3916363/f7b49678b883/pone.0087978.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8318/3916363/e767971c9bc8/pone.0087978.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8318/3916363/5ce094f476b3/pone.0087978.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8318/3916363/52b2853cc53c/pone.0087978.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8318/3916363/23aabbe356e4/pone.0087978.g004.jpg
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本文引用的文献

1
Comparative glycomic profiling of isotopically permethylated N-glycans by liquid chromatography/electrospray ionization mass spectrometry.利用液相色谱/电喷雾电离质谱技术对同位素全甲基化 N-糖进行糖组学比较分析。
Rapid Commun Mass Spectrom. 2013 Apr 30;27(8):865-77. doi: 10.1002/rcm.6512.
2
Cancer statistics, 2013.癌症统计数据，2013 年。
CA Cancer J Clin. 2013 Jan;63(1):11-30. doi: 10.3322/caac.21166. Epub 2013 Jan 17.
3
Quantitative glycomics strategies.定量糖组学策略。
Cancer Cell Int. 2022 Dec 19;22(1):414. doi: 10.1186/s12935-022-02832-3.
4
ALG3 Promotes Peritoneal Metastasis of Ovarian Cancer through Increasing Interaction of α1,3-mannosylated uPAR and ADAM8.ALG3 通过增加 α1,3-甘露糖化 uPAR 和 ADAM8 的相互作用促进卵巢癌细胞腹膜转移。
Cells. 2022 Oct 6;11(19):3141. doi: 10.3390/cells11193141.
5
In Situ -Glycosylation Signatures of Epithelial Ovarian Cancer Tissue as Defined by MALDI Mass Spectrometry Imaging.基质辅助激光解吸电离质谱成像定义的上皮性卵巢癌组织原位糖基化特征
Cancers (Basel). 2022 Feb 17;14(4):1021. doi: 10.3390/cancers14041021.
6
A Novel Glycosyltransferase-Related Gene Signature for Overall Survival Prediction in Patients with Ovarian Cancer.一种用于预测卵巢癌患者总生存期的新型糖基转移酶相关基因特征
Int J Gen Med. 2021 Dec 25;14:10337-10350. doi: 10.2147/IJGM.S332945. eCollection 2021.
7
Exosomal miR‑663b exposed to TGF‑β1 promotes cervical cancer metastasis and epithelial‑mesenchymal transition by targeting MGAT3.外泌体 miR-663b 在 TGF-β1 作用下促进宫颈癌转移和上皮间质转化，其作用靶点是 MGAT3。
Oncol Rep. 2021 Apr;45(4). doi: 10.3892/or.2021.7963. Epub 2021 Mar 2.
8
Quantitative clinical glycomics strategies: A guide for selecting the best analysis approach.定量临床糖组学策略：选择最佳分析方法的指南。
Mass Spectrom Rev. 2022 Nov;41(6):901-921. doi: 10.1002/mas.21688. Epub 2021 Feb 10.
9
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Clin Proteomics. 2020 Sep 19;17:34. doi: 10.1186/s12014-020-09297-4. eCollection 2020.
10
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Theranostics. 2020 May 16;10(14):6467-6482. doi: 10.7150/thno.43865. eCollection 2020.
Mol Cell Proteomics. 2013 Apr;12(4):874-84. doi: 10.1074/mcp.R112.026310. Epub 2013 Jan 16.
4
Quantitative analysis of glycoprotein glycans.糖蛋白聚糖的定量分析
Methods Mol Biol. 2013;951:197-215. doi: 10.1007/978-1-62703-146-2_13.
5
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Int J Biochem Cell Biol. 2013 Feb;45(2):347-58. doi: 10.1016/j.biocel.2012.10.006. Epub 2012 Oct 24.
6
Quantification of the N-glycosylated secretome by super-SILAC during breast cancer progression and in human blood samples.通过超级 SILAC 在乳腺癌进展过程中和人血样本中对 N-糖基化分泌组进行定量分析。
Mol Cell Proteomics. 2013 Jan;12(1):158-71. doi: 10.1074/mcp.M112.023614. Epub 2012 Oct 22.
7
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Curr Protoc Chem Biol. 2010 Apr 1;2:55-69. doi: 10.1002/9780470559277.ch090207.
8
Glycosylation effects on cancer development.糖基化对癌症发展的影响。
Glycoconj J. 2012 Dec;29(8-9):565-6. doi: 10.1007/s10719-012-9448-4.
9
Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells.人转移性肝癌细胞中与聚糖相关的基因表达特征
Exp Ther Med. 2012 Mar;3(3):415-422. doi: 10.3892/etm.2011.430. Epub 2011 Dec 23.
10
Regulation of the metastatic cell phenotype by sialylated glycans.唾液酸化糖缀合物对转移性细胞表型的调控。
Cancer Metastasis Rev. 2012 Dec;31(3-4):501-18. doi: 10.1007/s10555-012-9359-7.