Bukharin O V, Kremleva E A, Sgibnev A V
Zh Mikrobiol Epidemiol Immunobiol. 2012 Nov-Dec(6):89-95.
Study the role of associative microsymbionts in biocenosis based on comparative evaluation of interbacterial and epithelial-bacterial interactions on the example of vaginal biotope.
Corynebacterium spp., Staphylococcus aureus, Escherichia coli and Lactobacillus spp. and primary epitheliocytes isolated from the lower part of reproductive tract of women were used. Interactions by associant-macropartner vector were evaluated by changes of growth properties and antagonistic activity of the associant under the influence of exometabolites of vaginal epitheliocytes and expression of cytokines by epitheliocytes under the influence of associants. The nature of interaction by associant-dominant vector was evaluated by changes of adhesive properties and biofilm formation of associants and bactericidal activity of peroxide-producing lactobacilli.
Groups of associants that are opposite by their function in symbiosis were isolated. An example of associants that stabilize associative symbiosis by stimulating growth and antagonistic activity of dominant, moderate increase of cytokine production by epitheliocytes are corynebacteria, whose growth, biofilm formation, adhesion and antagonism during interaction with dominant and macropartner are also increased. The nature of interaction of associants of the other group (S. aureus and E. coli) with dominant and macropartner is the opposite. The determinative role of hydrogen peroxide in mechanisms of differentiation of associative microsymbionts and functioning of associative symbiosis ofvagina was shown.
The proposed approach allows to differentiate associative microsymbionts with various ecological roles, evaluate their contribution into maintenance of the stability of the symbiosis and open new possibilities of management of quality of microbiocenosis.
以阴道生物群落为例，通过对细菌间和上皮-细菌相互作用的比较评估，研究共生微共生体在生物群落中的作用。
使用从女性生殖道下部分离出的棒状杆菌属、金黄色葡萄球菌、大肠杆菌、乳杆菌属以及原代上皮细胞。通过共生体在阴道上皮细胞外代谢产物影响下生长特性和拮抗活性的变化，以及共生体影响下上皮细胞细胞因子的表达，评估共生体-大伙伴载体的相互作用。通过共生体黏附特性和生物膜形成的变化以及产过氧化氢乳杆菌的杀菌活性，评估共生体-优势载体的相互作用性质。
分离出了在共生中功能相反的共生体组。棒状杆菌就是通过刺激优势菌的生长和拮抗活性来稳定共生共生关系、使上皮细胞细胞因子产生适度增加的共生体实例，其在与优势菌和大伙伴相互作用过程中的生长、生物膜形成、黏附及拮抗作用也增强。另一组共生体（金黄色葡萄球菌和大肠杆菌）与优势菌和大伙伴的相互作用性质则相反。过氧化氢在阴道共生微共生体分化机制及共生共生功能中起决定性作用。
所提出的方法能够区分具有不同生态作用的共生微共生体，评估它们对维持共生稳定性的贡献，并为微生物群落质量管理开辟新的可能性。
