Kremleva E A, Sgibnev A V, Cherkasov S V
Zh Mikrobiol Epidemiol Immunobiol. 2015 Jul-Aug(4):75-9.
Study regularities of effects of various types of vaginal microsymbionts on the ability of mucosal epitheliocytes of the vagina to modify biological properties of bacteria.
Effect of thermo-inactivated cells of Staphylococcus aureus, Escherichia coli, H2O2-producing and H2O2-non-producing lactobacilli on the ability of primary vaginal epitheliocytes to alter growth and antagonistic activity of Staphylococcus aureus, Escherichia colt, H2O2-producing and H2O2-nonproducing lactobacilli was studied using a multi-component module system.
Alterations of composition of vaginal epitlieliocyte exometabolites under the effect of S. aureus and E. coli was established to result in a pronounced stimulation of antagonistic activity of H2O2-producing and an increase of the number of H2O2-nonproducing lactobacilli. Thermo-inactivated cells of lactobacilli stimulated production of metabolites by epitheliocytes, that suppress the growth and antagonistic activity of allochthonous microflora and stimulate similar parameters of lactobacilli. The strongest effects on the ability of vaginal epitheliocytes to alter biological properties of bacteria were rendered by H2O2-producing lactobacilli.
The regularities identified allow to examine epitheliocytes and normoflora of vagina as a symbiotic system, the coordinated interaction of its components is directed on maintaining microecological stability of female reproductive tract.
研究不同类型阴道微共生体对阴道黏膜上皮细胞改变细菌生物学特性能力的影响规律。
使用多组分模块系统，研究热灭活的金黄色葡萄球菌、大肠杆菌、产H2O2和不产H2O2的乳酸杆菌细胞对原代阴道上皮细胞改变金黄色葡萄球菌、大肠杆菌、产H2O2和不产H2O2的乳酸杆菌生长及拮抗活性能力的影响。
已确定在金黄色葡萄球菌和大肠杆菌作用下，阴道上皮细胞外代谢产物组成的改变会导致产H2O2乳酸杆菌的拮抗活性显著增强，以及不产H2O2乳酸杆菌数量增加。乳酸杆菌的热灭活细胞刺激上皮细胞产生代谢产物，这些代谢产物可抑制外来微生物群落的生长和拮抗活性，并刺激乳酸杆菌的类似参数。产H2O2乳酸杆菌对阴道上皮细胞改变细菌生物学特性的能力影响最强。
所确定的规律有助于将阴道上皮细胞和正常菌群作为一个共生系统进行研究，其组成部分的协同相互作用旨在维持女性生殖道的微生态稳定性。
