Kremleva E A, Cherkasov S V, Bukharin O V
Zh Mikrobiol Epidemiol Immunobiol. 2012 Nov-Dec(6):95-9.
Evaluate significance of epithelial-bacterial interactions for the formation and/or support of microbiocenoses.
Effect of vaginal epitheliocytes on growth properties of lactobacilli strains obtained from the same biotope from 16 women with vaginal normocenosis was studied. During intravaginal probiotic therapy in 24 women with vaginal dysbiosis probability of colonization of vagina by probiotic strain was evaluated depending on the result of effect of vaginal epitheliocytes on its growth properties.
Exometabolites of epitheliocytes in normocenosis were shown to render stimulating effect on the growth of autostrain of dominant microflora, that probably is the basis of formation and/or support of microbiocenosis. Stimulating effect of exometabolites of vaginal epitheliocytes of patients on growth properties of probiotic strain was revealed to be the prerequisite for successful probiotic therapy.
The data obtained allow to examine epithelial-bacterial interactions as a basis of formation of microbiocenoses. Evaluation of these interactions may be used for individual selection of probiotic strains.
评估上皮细胞与细菌相互作用对微生物群落形成和/或维持的意义。
研究了阴道上皮细胞对从16名阴道微生态正常的女性同一生物群落中分离出的乳酸杆菌菌株生长特性的影响。在对24名阴道生态失调的女性进行阴道内益生菌治疗期间，根据阴道上皮细胞对益生菌菌株生长特性的影响结果，评估益生菌菌株在阴道定植的可能性。
微生态正常时上皮细胞的外代谢产物对优势微生物群落自身菌株的生长具有刺激作用，这可能是微生物群落形成和/或维持的基础。患者阴道上皮细胞外代谢产物对益生菌菌株生长特性的刺激作用是益生菌治疗成功的先决条件。
所获得的数据有助于将上皮细胞与细菌的相互作用作为微生物群落形成的基础进行研究。对这些相互作用的评估可用于益生菌菌株的个体化选择。
