Sekhwal Manoj Kumar, Sharma Vinay, Sarin Renu
Department of Bioscience and Biotechnology, Banasthali University, P.O. Banasthali Vidyapith, 304022 Vanasthali, Rajasthan, India.
Theory Biosci. 2013 Jun;132(2):105-13. doi: 10.1007/s12064-012-0174-z. Epub 2013 Jan 9.
The antiporters, uniporters and symporters are the functional classes of MFS that play major role in ions homeostasis, regulation of pumps and channels, membrane structure, transporters activity in tolerance to abiotic stresses. Major facilitator superfamily (MFS) encodes Na(+)/H(+) antiporter that are considered as being sensors of the molecule transports. A large number of MFS proteins have been identified in several plants, rice, maize, Arabidopsis etc. However, the majority of proteins in sorghum are described as putative, uncharacterized till date. This suggested that identified proteins of MFS in sorghum are far from saturation. Hence, we developed gene ontology (GO) terms semantic similarity based method using GOSemSim measure of R package. As a result, total 2,568 high (100 %) semantic similar orthologous proteins from 7 plant species were obtained. These data were used to predict function of 257 putative uncharacterized proteins from 18 families of MFS in Sorghum. Consequently, the identified proteins belonged to the function of regulation of pumps and channels, membrane structure, transporters activity, ions homeostasis, transporter mechanisms and binding process. These identified functions appear to have a distinct mechanism of salt-stress adaptation in plants. The proposed method will help in further identifying new proteins that can help in the development of agronomically and economically important plants.
反向转运蛋白、单向转运蛋白和同向转运蛋白是主要协助转运超家族（MFS）的功能类别，在离子稳态、泵和通道的调节、膜结构以及植物对非生物胁迫耐受性中的转运蛋白活性方面发挥着重要作用。主要协助转运超家族（MFS）编码Na(+)/H(+)反向转运蛋白，这些蛋白被认为是分子运输的传感器。在几种植物中，如水稻、玉米、拟南芥等，已经鉴定出大量的MFS蛋白。然而，高粱中的大多数蛋白至今仍被描述为假定的、未表征的。这表明高粱中已鉴定的MFS蛋白远未饱和。因此，我们使用R包的GOSemSim度量开发了基于基因本体（GO）术语语义相似性的方法。结果，从7种植物中总共获得了2568个具有高（100%）语义相似性的直系同源蛋白。这些数据被用于预测高粱中18个MFS家族的257个假定未表征蛋白的功能。因此，鉴定出的蛋白属于泵和通道调节、膜结构、转运蛋白活性、离子稳态、转运机制和结合过程等功能类别。这些已鉴定的功能似乎在植物中具有独特的盐胁迫适应机制。所提出的方法将有助于进一步鉴定新的蛋白，这些蛋白有助于培育具有重要农艺和经济价值的植物。
