Department of Bioscience & Biotechnology, Banasthali University, P.O. Banasthali Vidyapith, 304022 Rajasthan, India.
Physiol Mol Biol Plants. 2012 Jul;18(3):209-16. doi: 10.1007/s12298-012-0121-y.
Sorghum bicolor (L.) is an important crop of arid and semi arid zones with most of its varieties tolerant to drought, heat and salt stress. Functional identification of many salt tolerant proteins has been reported in Arabidopsis, rice and other plants, however only little functional information has been predicted in sorghum till date. A 2-D gel electrophoresis based proteomic approach with MALDI-TOF mass spectrometer was utilized to analyze the salt stress response of sorghum. Major changes in protein complement were observed at 200 mM NaCl in hydroponic culture after 96 h of salt-stress. Highly expressed five proteins were excised for functional identification. We developed shortest path (SP) analysis based method on Gene Ontology (GO) hierarchy using sum of GO-term's semantic similarities. In this study, we observed that majority of expressed proteins belonged to the functional category of energy production and conversion, signal transduction mechanisms and ribosome maturation. These identified functions suggest a distinct mechanism of salt-stress adaptation in sorghum plant. The proposed method in this paper potentially has great importance to further understanding of newly identified proteins that can help in plant development.
高粱（Sorghum bicolor (L.)）是干旱和半干旱地区的重要作物，其大多数品种都能耐受干旱、高温和盐胁迫。已在拟南芥、水稻和其他植物中报道了许多耐盐蛋白的功能鉴定，但迄今为止，在高粱中仅预测了少量的功能信息。本研究利用基于 2-DE 电泳和 MALDI-TOF 质谱的蛋白质组学方法分析了高粱的盐胁迫反应。在水培培养中，96 小时盐胁迫后，在 200 mM NaCl 下观察到蛋白质组成的重大变化。对高表达的 5 种蛋白质进行了功能鉴定。我们使用基于 GO 层次结构的最短路径 (SP) 分析方法，计算了 GO 术语语义相似度的总和。本研究观察到，大多数表达的蛋白质属于能量产生和转化、信号转导机制和核糖体成熟等功能类别。这些鉴定出的功能表明高粱植物适应盐胁迫的机制独特。本文提出的方法对于进一步理解新鉴定的蛋白质具有重要意义，这有助于植物的发育。
