人乳头瘤病毒在阴茎癌中的病因学作用：越南的一项研究。
The etiologic role of human papillomavirus in penile cancers: a study in Vietnam.
机构信息
Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
出版信息
Br J Cancer. 2013 Jan 15;108(1):229-33. doi: 10.1038/bjc.2012.583. Epub 2013 Jan 8.
BACKGROUND
We investigated the aetiologic role of human papillomavirus (HPV) in 120 penile squamous cell carcinomas (PSCCs) from Vietnam.
METHODS
Human papillomavirus DNA was detected by PCR using SPF10 primers and a primer set targeting HPV-16 E6. The INNO-LiPA HPV genotyping kit was used to determine genotype. Human papillomavirus-16 viral load and physical status were determined by real-time PCR. P16(INK4A) protein expression was investigated by immunohistochemistry.
RESULTS
Human papillomavirus DNA was detected in 27 of 120 (23%) PSCCs. The most frequently detected genotype was HPV-16 (24 of 27 cases, 89%). In 16 of 18 (89%) HPV-16-positive cases, the HPV DNA was considered to be integrated into the host genome. The geometric mean of the HPV-16 viral load was 0.4 copies per cell. P16(INK4A) overexpression was significantly related to PSCCs infected with high-risk HPV (P=0.018) and HPV-16 copy numbers (P<0.001).
CONCLUSION
Human papillomavirus-16 DNA integration and p16(INK4A) overexpression in high-risk HPV detected PSCCs suggested an aetiologic role of high-risk HPV in the development of PSCCs.
背景
我们调查了人乳头瘤病毒（HPV）在 120 例越南阴茎鳞癌（PSCC）中的病因作用。
方法
采用 SPF10 引物和针对 HPV-16 E6 的引物组，通过 PCR 检测 HPV DNA。采用 INNO-LiPA HPV 基因分型试剂盒确定基因型。通过实时 PCR 检测 HPV-16 病毒载量和物理状态。通过免疫组化检测 P16(INK4A)蛋白表达。
结果
在 120 例 PSCC 中，有 27 例（23%）检测到 HPV DNA。最常检测到的基因型是 HPV-16（27 例中有 24 例，89%）。在 18 例 HPV-16 阳性病例中的 16 例（89%）中，HPV DNA 被认为已整合到宿主基因组中。HPV-16 病毒载量的几何平均值为每细胞 0.4 个拷贝。P16(INK4A)过表达与高危型 HPV 感染的 PSCC（P=0.018）和 HPV-16 拷贝数（P<0.001）显著相关。
结论
高危型 HPV 检测到的 HPV-16 DNA 整合和 P16(INK4A)过表达提示高危型 HPV 在 PSCC 发生发展中的病因作用。
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