Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark.
PLoS One. 2013;8(1):e53107. doi: 10.1371/journal.pone.0053107. Epub 2013 Jan 3.
Microfluidics systems usually consist of materials like PMMA--poly(methyl methacrylate) and PDMS--poly(dimethylsiloxane) and not polystyrene (PS), which is usually used for cell culture. Cellular and molecular responses in cells grown on PS are well characterized due to decades of accumulated research. In contrast, the experience base is limited for materials used in microfludics chip fabrication.
The effect of different materials (PS, PMMA and perforated PMMA with a piece of PDMS underneath) on the growth and differentiation of PC12 (adrenal phaeochromocytoma) cells into neuronal-like cells was investigated using cell viability, cell cycle distribution, morphology, and gene expression analysis.
RESULTS/CONCLUSIONS: After differentiation, the morphology, viability and cell cycle distribution of PC12 cells grown on PS, PMMA with and without PDMS underneath was the same. By contrast, 41 genes showed different expression for PC12 cells differentiating on PMMA as compared to on PS. In contrast, 677 genes showed different expression on PMMA with PDMS underneath as compared with PC12 cells on PS. The differentially expressed genes are involved in neuronal cell development and function. However, there were also many markers for neuronal cell development and functions that were expressed similarly in cells differentiating on PS, PMMA and PMMA with PDMS underneath. In conclusion, it was shown that PMMA has a minor impact and PDMS a major impact on gene expression in PC12 cells.
微流控系统通常由 PMMA（聚甲基丙烯酸甲酯）和 PDMS（聚二甲基硅氧烷）等材料组成，而不是通常用于细胞培养的聚苯乙烯（PS）。由于几十年的积累研究，PS 上生长的细胞的细胞和分子反应已得到很好的描述。相比之下，在微流控芯片制造中使用的材料的经验基础有限。
使用细胞活力、细胞周期分布、形态和基因表达分析，研究了不同材料（PS、PMMA 和 PMMA 下方的 PDMS 穿孔片）对 PC12（肾上腺嗜铬细胞瘤）细胞向神经元样细胞生长和分化的影响。
结果/结论：分化后，PS、PMMA 下方有无 PDMS 生长的 PC12 细胞的形态、活力和细胞周期分布相同。相比之下，与 PS 相比，在 PMMA 上分化的 PC12 细胞有 41 个基因的表达不同。相比之下，在 PMMA 下方有 PDMS 的情况下，有 677 个基因的表达与 PS 上的 PC12 细胞不同。差异表达的基因涉及神经元细胞的发育和功能。然而，在 PS、PMMA 和 PMMA 下方有 PDMS 上分化的细胞中，也有许多神经元细胞发育和功能的标志物表达相似。总之，结果表明 PMMA 对 PC12 细胞的基因表达影响较小，而 PDMS 影响较大。
