Khnouf Ruba, Karasneh Dina, Albiss Borhan Aldeen
Department of Biomedical Engineering, Faculty of Engineering, Jordan University of Science and Technology, Irbid, Jordan.
Department of Applied Physics, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan.
Electrophoresis. 2016 Feb;37(3):529-35. doi: 10.1002/elps.201500333. Epub 2015 Dec 11.
PDMS and PMMA are two of the most used polymers in the fabrication of lab-on-chip or microfluidic devices. In order to use these polymers in biological applications, it is sometimes essential to be able to bind biomolecules such as proteins and DNA to the surface of these materials. In this work, we have evaluated a number of processes that have been developed to bind protein to PDMS surfaces which include passive adsorption, passive adsorption with glutaraldehyde cross-linking, (3-aminopropyl) triethoxysilane functionalization followed by glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride cross-linkers. It has been shown that the latter technique--using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride--results in more than twice the bonding of protein to the surface of PDMS microchannels than proteins binding passively. We have also evaluated a few techniques that have been tested for the functionalization of PMMA microchannels where we have found that the use of polyethyleneimine (PEI) has led to the strongest protein-PMMA microchannel bond. We finally demonstrated the effect of PDMS curing methodology on protein adsorption to its surface, and showed that increased curing time is the factor that reduces passive adsorption the most.
聚二甲基硅氧烷（PDMS）和聚甲基丙烯酸甲酯（PMMA）是在芯片实验室或微流控设备制造中最常用的两种聚合物。为了在生物应用中使用这些聚合物，有时能够将蛋白质和DNA等生物分子结合到这些材料的表面至关重要。在这项工作中，我们评估了一些已开发的将蛋白质结合到PDMS表面的方法，包括被动吸附、用戊二醛交联的被动吸附、（3-氨丙基）三乙氧基硅烷功能化，然后使用戊二醛或1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐交联剂。结果表明，后一种技术——使用1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐——使蛋白质与PDMS微通道表面的结合量比被动结合的蛋白质多出两倍以上。我们还评估了一些已测试用于PMMA微通道功能化的技术，发现使用聚乙烯亚胺（PEI）可导致蛋白质与PMMA微通道之间的结合最强。我们最终证明了PDMS固化方法对蛋白质吸附到其表面的影响，并表明固化时间的增加是最能减少被动吸附的因素。
