印度北部一家三级护理中心的腹泻患者中分离出的弯曲杆菌属菌种。

Campylobacter species associated with diarrhoea in patients from a tertiary care centre of north India.

作者信息

Prasad K N, Dixit A K, Ayyagari A

机构信息

Department of Microbiology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow, India.

出版信息

Indian J Med Res. 2001 Jul;114:12-7.

PMID:11762201

Abstract

BACKGROUND & OBJECTIVES: Most laboratories do not routinely distinguish the various Campylobacter species, though almost all Campylobacter species have been isolated from human faeces. The epidemiological and clinical aspects of its infection and the species involved in genesis of diarrhoea are least understood in the developing countries. The aim of the present study was to find out frequency of Campylobacter species isolated from patients with diarrhoea over a 12-year period and to analyse their features.

METHODS

Campylobacter strains isolated from stool samples of patients with diarrhoea were identified to the species level on appropriate media at 42 degrees C micro-aerobically. Patients' demography and clinical data were analyzed retrospectively; 25 Campylobacter jejuni strains were tested for toxin production and 23 strains were typed by Penner scheme.

RESULTS

A total of 62 strains were isolated from 59 patients and the various species were C. jejuni 51 (82.3%), C. coli 8 (12.9%), C. lari 2 (3.2%), and C. upsaliensis 1 (1.6%). Children < 5 yr of age were most affected (34/59; 57.6%), followed by patients in 15-30 yr of age (12/59; 20.3%). Presentation of watery diarrhoea was significantly more common than inflammatory diarrhoea (50/59, 84.7% vs 9/59, 15.3%; P < 0.001). Recurrence occurred in 3 (5.1%) patients. Majority of the infections resolved within one week; one HIV-positive patient had chronic diarrhoea. Two patients developed Guillain-Barré syndrome following Campylobacter infection. Twenty (80%) of 25 strains were toxigenic and 20 (87%) of 23 strains could be typed by Penner scheme.

INTERPRETATION & CONCLUSION: In our patients, 4 different Campylobacter species and various C. jejuni serotypes were involved in gastroenteritis. Majority of the infections were watery diarrhoea and in children < 5 yr of age. There is a need of a population-based systematic study to know the epidemiology of whole spectrum of campylobacters in India.

摘要

背景与目的

尽管几乎所有弯曲杆菌属菌种都已从人类粪便中分离出来，但大多数实验室并不常规区分不同的弯曲杆菌属菌种。在发展中国家，人们对其感染的流行病学和临床情况以及与腹泻发生相关的菌种了解最少。本研究的目的是查明在12年期间从腹泻患者中分离出的弯曲杆菌属菌种的频率，并分析其特征。

方法

将从腹泻患者粪便样本中分离出的弯曲杆菌菌株在42摄氏度微需氧条件下于合适培养基上鉴定到菌种水平。对患者的人口统计学和临床数据进行回顾性分析；对25株空肠弯曲杆菌菌株进行毒素产生检测，并用Penner分型法对23株菌株进行分型。

结果

共从59例患者中分离出62株菌株，不同菌种分别为空肠弯曲杆菌51株（82.3%）、大肠弯曲杆菌8株（12.9%）、拉氏弯曲杆菌2株（3.2%）和乌普萨拉弯曲杆菌1株（1.6%）。5岁以下儿童受影响最大（34/59；57.6%），其次是15至30岁的患者（12/59；20.3%）。水样腹泻的表现明显比炎性腹泻更常见（50/59，84.7%对9/59，15.3%；P<0.001）。3例（5.1%）患者出现复发。大多数感染在一周内痊愈；1例艾滋病毒阳性患者患有慢性腹泻。2例患者在弯曲杆菌感染后发生格林-巴利综合征。25株菌株中有20株（80%）产毒素，23株菌株中有20株（87%）可用Penner分型法分型。