Rebaud Samuel, Maniti Ofelia, Girard-Egrot Agnès P
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1, University of Lyon, ICBMS, CNRS UMR 5246, GEMBAS Team, Bât. Curien, 43 bd du 11 Nov. 1918, F-69622 Villeurbanne Cedex, France.
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1, University of Lyon, ICBMS, CNRS UMR 5246, GEMBAS Team, Bât. Curien, 43 bd du 11 Nov. 1918, F-69622 Villeurbanne Cedex, France.
Biochimie. 2014 Dec;107 Pt A:135-42. doi: 10.1016/j.biochi.2014.06.021. Epub 2014 Jul 4.
Biological membranes play a central role in the biology of the cell. They are not only the hydrophobic barrier allowing separation between two water soluble compartments but also a supra-molecular entity that has vital structural functions. Notably, they are involved in many exchange processes between the outside and inside cellular spaces. Accounting for the complexity of cell membranes, reliable models are needed to acquire current knowledge of the molecular processes occurring in membranes. To simplify the investigation of lipid/protein interactions, the use of biomimetic membranes is an approach that allows manipulation of the lipid composition of specific domains and/or the protein composition, and the evaluation of the reciprocal effects. Since the middle of the 80's, lipid bilayer membranes have been constantly developed as models of biological membranes with the ultimate goal to reincorporate membrane proteins for their functional investigation. In this review, after a brief description of the planar lipid bilayers as biomimetic membrane models, we will focus on the construction of the tethered Bilayer Lipid Membranes, the most promising model for efficient membrane protein reconstitution and investigation of molecular processes occurring in cell membranes.
生物膜在细胞生物学中起着核心作用。它们不仅是允许两个水溶性区室分离的疏水屏障，还是具有重要结构功能的超分子实体。值得注意的是，它们参与细胞内外空间之间的许多交换过程。考虑到细胞膜的复杂性，需要可靠的模型来获取目前关于膜中发生的分子过程的知识。为了简化对脂质/蛋白质相互作用的研究，使用仿生膜是一种允许操纵特定结构域的脂质组成和/或蛋白质组成，并评估相互作用效应的方法。自80年代中期以来，脂质双分子层膜一直作为生物膜模型不断发展，其最终目标是重新纳入膜蛋白以进行功能研究。在这篇综述中，在简要描述平面脂质双分子层作为仿生膜模型之后，我们将重点关注栓系双分子层脂质膜的构建，这是用于高效膜蛋白重构和研究细胞膜中发生的分子过程的最有前景的模型。
