Department of Biotechnology, Sathyabama University, Chennai-600 119, India.
Chin J Nat Med. 2012 Jan;10(1):77-80. doi: 10.1016/S1875-5364(12)60017-X.
Mangrove is one of the oldest living tree species and its leaves are among the most extensively studied botanicals in use today. Scientific research throughout the world has found evidence to support the fact that its foliar extracts have great potential against human microbial pathogens. This study highlights the isolation of foliar fungi from Rhizophora mucronata, Avicenna officialis and Avicenna marina.
It was isolated in Sabouroud's Dextrose Agar and mass cultivation was done in Sabouroud's Dextrose broth.
The ethyl acetate extract showed maximum antibacterial activity which inturn checked for different concentration against bacterial pathogens and anticancer activity for Hep2 and MCF7 cell line in vitro. The DNA was isolated from the fungi and the ITS region of 5.8 s RNA was sequenced and assigned to new species as they are separated from the type strains phylogenetic neighbors by sequence similarities.
This preliminary screening of fungal endophytes revealed their potential to yield potent bioactive compounds for drug discovery programmes.
红树林是最古老的树种之一，其叶子是当今应用最广泛的植物药之一。世界各地的科学研究都有证据表明，其叶提取物对人类微生物病原体具有巨大的潜力。本研究从红树、官桂和海杧果中分离叶真菌。
在萨布罗葡萄糖琼脂中进行分离，并在萨布罗葡萄糖肉汤中进行大规模培养。
乙酸乙酯提取物表现出最大的抗菌活性，继而针对不同浓度的细菌病原体进行了检查，并在体外对 Hep2 和 MCF7 细胞系进行了抗癌活性检测。从真菌中分离出 DNA，对 5.8 s RNA 的 ITS 区进行测序，并将其分配到新物种中，因为它们与模式株的系统发育邻居通过序列相似性而分离。
本研究对真菌内生菌的初步筛选表明，它们有潜力产生用于药物发现计划的有效生物活性化合物。
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