Rahaman Md Sohanur, Siraj Md Afjalus, Sultana Sabiha, Seidel Veronique, Islam Md Amirul
Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh.
Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI, United States.
Front Microbiol. 2020 Nov 13;11:570855. doi: 10.3389/fmicb.2020.570855. eCollection 2020.
The Sundarbans forest in Bangladesh is the world's largest mangrove. It is a unique ecosystem where living organisms face extreme challenges to compete for survival. Such competition results in the production of bioactive molecules which are useful for agriculture and human health. In this study, eighty fungal endophytes from nine mangrove plants growing in a region, as yet unexplored, of the Sundarbans were isolated by surface sterilisation and pure culture techniques. Among the eighty isolates subjected to a preliminary antimicrobial screening using an agar plug diffusion assay, only fifteen showed some promising activity. These were subsequently identified by polymerase chain reaction of their ITS gene. Extracts prepared from the identified isolates were screened for antimicrobial, antioxidant, cytotoxic and α-glucosidase inhibitory activities. Their total polyphenol and flavonoid content and their FRAP value were also determined. All endophytes are reported for the first time in the plants under investigation.
孟加拉国的孙德尔本斯森林是世界上最大的红树林。它是一个独特的生态系统，其中的生物在争夺生存的过程中面临着极端挑战。这种竞争导致了生物活性分子的产生，这些分子对农业和人类健康有益。在本研究中，通过表面消毒和纯培养技术，从孙德尔本斯一个尚未被探索的区域生长的九种红树林植物中分离出了八十种真菌内生菌。在使用琼脂块扩散法进行初步抗菌筛选的八十个分离株中，只有十五个表现出了一些有前景的活性。随后通过其ITS基因的聚合酶链反应对它们进行了鉴定。对从鉴定出的分离株中制备的提取物进行了抗菌、抗氧化、细胞毒性和α-葡萄糖苷酶抑制活性的筛选。还测定了它们的总多酚和黄酮含量以及它们的FRAP值。所有内生菌均首次在被研究的植物中被报道。
