Broccardo M
Institute of Pharmacology, University La Sapienza, Rome, Italy.
Pharmacol Res. 1990 Mar-Apr;22(2):189-96. doi: 10.1016/1043-6618(90)90715-p.
Sauvagine is a 40 amino acid peptide, isolated from the skin of the South American frog, Phyllomedusa sauvagei, and structurally related to mammalian corticotropin releasing factor (CRF). Experiments with centrally or peripherally injected sauvagine produced dose-dependent hypothermia in rats kept at ambient temperatures of +4 degrees C and +22 degrees C, but no effect was seen on rats maintained at +34 degrees C. To determine whether a pituitary-adrenal dependent step was involved, the thermoregulatory response was studied in hypophysectomized, adrenalectomized and sham-operated rats maintained at a room temperature of +22 degrees C. Significant hypothermia was produced in all three experimental groups, showing that this response is not mediate by the pituitary-adrenal axis. CRF had no effect on the thermoregulatory functions of rats and furthermore alpha-helical CRF (9-41), a CRF receptor antagonist, which inhibited the pituitary and cardiovascular actions of sauvagine, did not modify the thermoregulatory response in rats.
蛙皮素是一种由40个氨基酸组成的肽，从南美蛙皮膜蛙（Phyllomedusa sauvagei）的皮肤中分离出来，在结构上与哺乳动物促肾上腺皮质激素释放因子（CRF）相关。在环境温度为+4℃和+22℃的大鼠中，经中枢或外周注射蛙皮素的实验产生了剂量依赖性体温过低，但在+34℃饲养的大鼠中未观察到影响。为了确定是否涉及垂体-肾上腺依赖步骤，在室温为+22℃的垂体切除、肾上腺切除和假手术大鼠中研究了体温调节反应。所有三个实验组均出现显著体温过低，表明该反应不是由垂体-肾上腺轴介导的。CRF对大鼠的体温调节功能没有影响，此外，α-螺旋CRF（9-41）是一种CRF受体拮抗剂，可抑制蛙皮素的垂体和心血管作用，但并未改变大鼠的体温调节反应。
