Broccardo M, Improta G
Institute of Pharmacology III, University La Sapienza, Rome, Italy.
Eur J Pharmacol. 1990 Jul 3;182(2):357-62. doi: 10.1016/0014-2999(90)90294-g.
Sauvagine and CRF significantly delayed gastric emptying in rats: sauvagine was 21 times more potent than CRF in inhibiting gastric emptying when injected subcutaneously and 59 times more potent when injected intracerebroventricularly. The inhibitory effect of intracerebroventricular and subcutaneous injections of sauvagine and CRF was always reversed by vagotomy. Hypophysectomy significantly reduced the inhibitory effect of subcutaneously injected sauvagine and CRF, but not that of intracerebroventricularly injected peptides. Adrenalectomy completely prevented the inhibition of gastric emptying only if it was performed 7 days before peptide administration, whereas the central and peripheral inhibitory effect of sauvagine and CRF was retained after acute (1 h) adrenalectomy. In animals adrenalectomized 7 days previously, chronic administration or a single dose of corticosterone at different intervals (1, 24 and 72 h) before the test caused the peptides to recover their inhibitory activity. These results demonstrate that the inhibitory action of sauvagine and CRF clearly depends on the integrity of the parasympathetic nervous system (vagus), and on corticosterone secretion by the adrenal gland.
蛙皮素和促肾上腺皮质激素释放因子（CRF）可显著延缓大鼠胃排空：皮下注射时，蛙皮素抑制胃排空的效力比CRF强21倍；脑室内注射时，蛙皮素的效力比CRF强59倍。迷走神经切断术总能逆转脑室内和皮下注射蛙皮素及CRF的抑制作用。垂体切除显著降低了皮下注射蛙皮素和CRF的抑制作用，但对脑室内注射肽类的抑制作用没有影响。仅当在给予肽类前7天进行肾上腺切除术时，肾上腺切除术才完全阻止了胃排空的抑制，而急性（1小时）肾上腺切除术后，蛙皮素和CRF的中枢和外周抑制作用仍保留。在7天前进行肾上腺切除术的动物中，在测试前不同时间间隔（1、24和72小时）长期给药或单次给予皮质酮可使肽类恢复其抑制活性。这些结果表明，蛙皮素和CRF的抑制作用明显依赖于副交感神经系统（迷走神经）的完整性以及肾上腺皮质酮的分泌。
