Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.
J Biol Eng. 2013 Jan 10;7(1):2. doi: 10.1186/1754-1611-7-2.
There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.
人们对强大的 DNA 组装策略的需求日益增长，以便快速、准确地构建用于合成生物学的遗传回路。这项技术的一个应用是重新构建多基因组装。在这里，我们整合了一个新的软件工具链和 2ab 组装，并表明它具有足够的鲁棒性，可以生成 528 个不同的复合部件，无错误的成功率为 96%。最后，我们根据其对生物安全和生物安保的影响讨论了我们的发现。
