Evans D H, Weingarten K
Center for Membrane Toxicity Studies, Mt. Desert Island Biological Laboratory, Salsbury Cove, ME 04672.
Toxicology. 1990 Apr 30;61(3):275-81. doi: 10.1016/0300-483x(90)90177-i.
The effect of Cd2+ and related metals (Ni2+, Hg2+, Pb2+, Co2+, Sn2+ Cu2+ and Zn2+) on vascular tension was studied using isolated rings of endothelium-free, smooth muscle from the ventral aorta of the shark, Squalus acanthias. Both Cd2+ and Ni2+ produced significant vasoconstriction at concentrations at or above 10(-6) M (112 and 59 ppb, respectively); the other metals were either marginally constrictive (Hg2+ and Sn2+) or were without effect (Pb2+, Co2+, Cu2+, and Zn2+). We suggest that previously published vascular effects of Hg2+ and Pb2+ may have been secondary to responses of the vascular endothelium, and that the role of Ni2+ in hypertension should be investigated further. Our data indicate that the effects of metals on this vascular smooth muscle are specific and not generic. Moreover, this system could be utilized to investigate the mechanisms of metal-induced vasoconstriction.
利用姥鲨（Squalus acanthias）腹主动脉无内皮的平滑肌分离环，研究了镉离子（Cd2+）及相关金属离子（镍离子（Ni2+）、汞离子（Hg2+）、铅离子（Pb2+）、钴离子（Co2+）、锡离子（Sn2+）、铜离子（Cu2+）和锌离子（Zn2+））对血管张力的影响。Cd2+和Ni2+在浓度达到或高于10^(-6) M（分别为112 ppb和59 ppb）时均产生显著的血管收缩作用；其他金属离子要么仅有轻微的收缩作用（Hg2+和Sn2+），要么无作用（Pb2+、Co2+、Cu2+和Zn2+）。我们认为，先前发表的Hg2+和Pb2+对血管的作用可能继发于血管内皮的反应，并且Ni2+在高血压中的作用应进一步研究。我们的数据表明，金属离子对这种血管平滑肌的作用具有特异性而非一般性。此外，该系统可用于研究金属诱导血管收缩的机制。
