Departamento de Oftalmologia, Otorrinolaringologia e CIrurgia de Cabeça e Pescoço Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes no. 3900, Ribeirão Preto - SP, Brazil.
Braz J Otorhinolaryngol. 2012 Dec;78(6):47-50. doi: 10.5935/1808-8694.20120032.
There is evidence that a "resistance phenomenon" occurs when a none-damaging dose of amikacin protects the hair cells from ototoxicity. Our goal is to prove that this resistance is persistent.
Experimental study - 14 albino guinea pigs (Cavia porcellus) divided into three groups. The auditory function was assessed by distortion product otoacoustic emissions (DPOAE): before exposure to amikacin, on the 15th day after the non-damaging dose was injected, at the end of the damage dose injection and prior to decapitation.
Group A (control) presented normal hearing and histological pattern. Group B (amikacin 20mg/kg/day (IM) for 30 days and affecting dose (400 mg / kg / day) for 12 days and Group C (same protocol of Group B, but kept for 60 days and slaughtered), the DPOAE confirmed normal auditory function in the pre-exposure and maintenance of the standard-dose; however, significant loss of auditory function after the end of the damaging dose injection.
The protection phenomenon did not extended for a period of 30 to 60 days after the application of damaging doses of amykacin.
有证据表明，当阿米卡星的非损伤剂量保护毛细胞免受耳毒性时，会出现“耐药现象”。我们的目标是证明这种耐药性是持久的。
实验研究-14 只白化豚鼠（豚鼠）分为三组。通过畸变产物耳声发射（DPOAE）评估听觉功能：在接触阿米卡星之前、注射非损伤剂量后第 15 天、损伤剂量注射结束前和断头前。
A 组（对照组）听力和组织学模式正常。B 组（20mg/kg/天（IM）阿米卡星 30 天，损伤剂量（400mg/kg/天）12 天）和 C 组（与 B 组相同方案，但保持 60 天并宰杀），DPOAE 在暴露前和标准剂量维持时证实听觉功能正常；然而，在损伤剂量注射结束后，听觉功能显著丧失。
在应用阿米卡星损伤剂量后的 30 至 60 天内，保护现象并未延长。
