Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirão Preto Medical School-FMRP, University of São Paulo-USP, Brazil.
Braz J Otorhinolaryngol. 2013 May-Jun;79(3):342-8. doi: 10.5935/1808-8694.20130060.
Hyperbaric oxygen therapy (HBOT) has enhanced the prevention and treatment of auditory ailments such as ototoxicity.
To study the effects of HBOT upon ototoxic injuries produced by amikacin.
This experimental study included 12 albino guinea pigs, whose auditory function was assessed through distortion product otoacoustic emissions (DPOAEs) and brainstem auditory evoked potentials (BAEPs) before and after the administration of amikacin (600 mg/kg/day) and HBOT sessions (2 ATA, 60 minutes). Morphological features were analyzed through scanning electron microscopy. Subjects were divided into four groups, as follows: group 1 - saline solution + HBOT; group 2 - amikacin for 8 days; group 3 - amikacin + seven days of rest; and group 4 - amikacin + HBOT.
Group 1 subjects had preserved function and morphology throughout the experiment; Group 2 subjects had statistically significant levels of hair cell injury and functional impairment; Subjects on groups 3 and 4 had statistically significant functional and morphological impairment after the administration of amikacin, which were still present after the proposed procedures had been carried out.
Hyperbaric oxygen therapy did not change the cochlear hair cell morphology or the electro-physiological thresholds of the guinea pigs given amikacin.
高压氧疗法（HBOT）增强了对听觉疾病（如耳毒性）的预防和治疗。
研究高压氧疗法对阿米卡星引起的耳毒性损伤的影响。
本实验研究包括 12 只白化豚鼠，在给予阿米卡星（600mg/kg/天）和高压氧治疗（2ATA，60 分钟）前后，通过畸变产物耳声发射（DPOAEs）和脑干听觉诱发电位（BAEPs）评估其听觉功能。通过扫描电子显微镜分析形态特征。将研究对象分为四组：组 1 - 生理盐水+高压氧；组 2 - 阿米卡星 8 天；组 3 - 阿米卡星+7 天休息；组 4 - 阿米卡星+高压氧。
组 1 研究对象在整个实验过程中功能和形态保持完好；组 2 研究对象毛细胞损伤和功能障碍程度具有统计学意义；组 3 和组 4 研究对象在给予阿米卡星后功能和形态均有明显损伤，即使在提出的方案实施后仍存在。
高压氧治疗未改变给予阿米卡星的豚鼠耳蜗毛细胞形态或电生理阈值。
