Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo 315211, China.
Med Oncol. 2013 Mar;30(1):365. doi: 10.1007/s12032-012-0365-y. Epub 2013 Jan 10.
MicroRNAs (miRNAs) play crucial roles during the occurrence and development of gastric cancer. Conventional serological tests for screening gastric cancer have limits on sensitivity and specificity. Several miRNAs in peripheral blood have been used as biomarkers of gastric cancer. However, most of these miRNAs are shared by several types of cancer. Thanks to the tissue specificity of gastric juice, here we examined the feasibility of using gastric juice miR-129-1/2, which are aberrantly expressed in gastric cancer, to screen gastric cancer. Total of 141 gastric juices samples from gastric cancer, gastric ulcer, atrophic gastritis, and minimal gastritis patients or subjects with normal mucosa were collected by gastroscopy. The gastric juice miR-129-1/2 levels were detected by quantitative reverse transcription-polymerase chain reaction. A receiver operating characteristic (ROC) curve was constructed for differentiating patients with gastric cancer from patients with benign gastric diseases. We showed that, compared with patients with benign gastric diseases, patients with gastric cancer had significantly lower levels of gastric juice miR-129-1-3p and miR-129-2-3p. The areas under ROC curve (AUC) were 0.639 and 0.651 for miR-129-1-3p and miR-129-2-3p, respectively. Using the parallel combination test, the AUC was up to 0.656. In summary, our results suggest that gastric juice miR-129-1-3p and miR-129-2-3p are potential biomarkers for the screening gastric cancer, and the detection of gastric juice miRNAs is a convenient non-invasion method for the diagnosis of gastric cancer.
微小 RNA（miRNAs）在胃癌的发生和发展中起着至关重要的作用。传统的用于筛查胃癌的血清学检测在灵敏度和特异性方面存在局限性。外周血中的几种 miRNAs 已被用作胃癌的生物标志物。然而，这些 miRNAs 中的大多数在几种癌症中都有表达。由于胃液具有组织特异性，我们在此研究了使用在胃癌中异常表达的胃液 miR-129-1/2 筛查胃癌的可行性。通过胃镜共采集了 141 例胃癌、胃溃疡、萎缩性胃炎和轻度胃炎患者以及正常黏膜患者的胃液样本。通过定量逆转录聚合酶链反应检测胃液 miR-129-1/2 水平。构建了区分胃癌患者和良性胃部疾病患者的受试者工作特征（ROC）曲线。我们发现，与良性胃部疾病患者相比，胃癌患者的胃液 miR-129-1-3p 和 miR-129-2-3p 水平显著降低。miR-129-1-3p 和 miR-129-2-3p 的 ROC 曲线下面积（AUC）分别为 0.639 和 0.651。使用平行组合检验，AUC 可达 0.656。综上所述，我们的研究结果表明，胃液 miR-129-1-3p 和 miR-129-2-3p 是筛查胃癌的潜在生物标志物，检测胃液 miRNAs 是诊断胃癌的一种方便的非侵入性方法。
