State Key Laboratory of Cancer Biology, Department of Gastrointestinal Surgery, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an 710032, Shanxi, People's Republic of China.
Med Oncol. 2013 Mar;30(1):421. doi: 10.1007/s12032-012-0421-7. Epub 2013 Jan 10.
We had reported that N-myc downstream-regulated gene (NDRG2) regulates colorectal cancer, breast cancer, clear cell renal cell carcinoma, pancreatic cancer, thyroid cancer and esophageal squamous cell proliferation, development, and apoptosis. The goal of this study was to determine the expression pattern of NDRG2 in human lung cancer and its correlation with prognosis. Immunohistochemistry, RT-PCR and western blot were used to explore the expression of NDRG2 in 185 human lung cancer patients. The correlation of NDRG2 expression with patients' survival rate was assessed by Kaplan-Meier and Cox regression. Results showed that the expression level of NDRG2 was decreased in human lung cancer tissues, and NDRG2 was positively correlated with depth of invasion (P = 0.038), vascular invasion (P = 0.036), tumor grade (P = 0.039), and tumor size (P = 0.026). Both RT-PCR and Western blots demonstrated that NDRG2 mRNA and protein levels were lower in lung cancer compared to the adjacent normal tissue from the same individual, and NDRG2 level was negatively correlated with UICC stage. Additionally, survival time of lung cancer patients with high expression of NDRG2 was longer than those with low expression during the 5-year follow-up period (P = 0.001). Meanwhile, COX regression analysis indicated that low expression of NDRG2, ≥pT(3), pM(1), ≥pN(1) and vascular invasion were independent, poor prognostic factors of lung cancer patients. These data showed that NDRG2 may play an important role in human lung cancer tumourigenesis, and NDRG2 might serve as a novel prognostic marker in human lung cancer.
我们曾报道过 N- 原肌球蛋白下游调节基因（NDRG2）可调节结直肠癌、乳腺癌、透明细胞肾细胞癌、胰腺癌、甲状腺癌和食管鳞状细胞的增殖、发育和凋亡。本研究旨在确定 NDRG2 在人肺癌中的表达模式及其与预后的关系。免疫组织化学、RT-PCR 和 Western blot 用于检测 185 例人肺癌患者中 NDRG2 的表达。通过 Kaplan-Meier 和 Cox 回归评估 NDRG2 表达与患者生存率的相关性。结果显示，NDRG2 在人肺癌组织中的表达水平降低，NDRG2 与浸润深度（P = 0.038）、血管侵犯（P = 0.036）、肿瘤分级（P = 0.039）和肿瘤大小（P = 0.026）呈正相关。RT-PCR 和 Western blot 均显示，与同一患者的相邻正常组织相比，肺癌组织中 NDRG2 mRNA 和蛋白水平均较低，且 NDRG2 水平与 UICC 分期呈负相关。此外，在 5 年随访期间，NDRG2 高表达的肺癌患者的生存时间长于 NDRG2 低表达的患者（P = 0.001）。同时，COX 回归分析表明，NDRG2 低表达、≥pT（3）、pM（1）、≥pN（1）和血管侵犯是肺癌患者独立的、预后不良的因素。这些数据表明，NDRG2 可能在人肺癌的肿瘤发生中发挥重要作用，NDRG2 可能成为人肺癌的一种新的预后标志物。
