Department of Chemistry, Capital Normal University, Beijing, China.
Electrophoresis. 2013 Mar;34(6):841-5. doi: 10.1002/elps.201200516. Epub 2013 Feb 18.
This article describes a new application of graphene oxide (GO) in CE based on the coating of fused silica capillary for chiral separation. The coated capillary was characterized by SEM, energy dispersive X-ray spectroscopy, and Raman spectra. The results indicated that the capillary was coated with GO. Chiral separations were carried out in the GO-coated capillary for the ephedrine-pseudoephedrine (E-PE) isomers and β-methylphenethylamine (β-Me-PEA) isomers at the optimal buffer conditions without any chiral selector by CE. The precision and reproducibility of GO-coated capillary were investigated, and the RSDs of migration time (n = 6) for the E-PE isomers were 1.35-1.41%, and 0.97-3.50% for β-Me-PEA isomers, respectively. The LOD for E-PE isomers and β-Me-PEA isomers was 3 μM and 18 μM, respectively.
本文描述了氧化石墨烯（GO）在基于涂层熔融石英毛细管的 CE 中的一种新应用，用于手性分离。通过 SEM、能谱和拉曼光谱对涂覆的毛细管进行了表征。结果表明，毛细管表面涂覆有 GO。在最优缓冲条件下，无需任何手性选择剂，通过 CE 对麻黄碱-伪麻黄碱（E-PE）异构体和β-甲基苯乙胺（β-Me-PEA）异构体在 GO 涂层毛细管中进行了手性分离。考察了 GO 涂层毛细管的精密度和重现性，E-PE 异构体的迁移时间（n = 6）的 RSD 分别为 1.35-1.41%，β-Me-PEA 异构体的 RSD 分别为 0.97-3.50%。E-PE 异构体和β-Me-PEA 异构体的 LOD 分别为 3 μM 和 18 μM。
