Department of Chemistry, Capital Normal University, Beijing, PR China.
J Sep Sci. 2013 Aug;36(16):2698-702. doi: 10.1002/jssc.201300304. Epub 2013 Jul 19.
A novel CE method was developed for the separation and determination of three main tropane alkaloids in Flos daturae with a capillary coated by graphene oxide (GO). The GO-coated capillary was characterized by SEM, energy dispersive X-ray spectroscopy, and Raman spectroscopy, and the results indicated that the inner surface of the capillary was partially coated by GO. A phosphate solution (40 mM, pH7.0) containing 20% v/v methanol and 30% v/v acetonitrile was used as the running buffer for the analysis of the atropine, scopolamine, and anisodamine. The linear ranges of atropine, scopolamine, and anisodamine was 0.5-200 μg/mL with satisfactory correlation coefficients (R(2)) > 0.9987, and this novel method provided an efficient separation for three tropane alkaloids as well as a good reproducibility and stability. Finally, the method was successfully applied for the determination of these three tropane alkaloids in plant extracts.
一种新型 CE 方法被开发用于分离和测定三种主要托烷生物碱在洋金花与毛细管涂覆氧化石墨烯（GO）。GO 涂覆的毛细管的特点是通过 SEM，能谱和拉曼光谱，结果表明，毛细管的内表面部分涂覆 GO。磷酸盐溶液（40 毫米，pH7.0）含有 20%v/v 甲醇和 30%v/v 乙腈作为运行缓冲液用于分析阿托品，莨菪碱和山莨菪碱。线性范围为 0.5-200μg/mL 与令人满意的相关系数（R（2））>0.9987，这种新方法提供了一种有效的分离三种托烷生物碱以及良好的重现性和稳定性。最后，该方法成功地应用于测定这些三种托烷生物碱在植物提取物。
